Japanese Journal of Veterinary Research;Volume 28, Number 4

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PRELIMINARY APPLICATION OF A FORMALIN FIXED TISSUE SECTION TO THE INDIRECT FLUORESCENT ANTIBODY TEST AND INTRAOVAL PRECIPITIN REACTION FOR THE DIAGNOSIS OF SCHISTOSOMIASIS JAPONICA

KAMIYA, Haruo;KAMIYA, Masao

Permalink : http://hdl.handle.net/2115/2209
JaLCDOI : 10.14943/jjvr.28.4.155

Abstract

A formalin fixed tissue section was applied to the indirect immunofluo-rescent antibody test (IFAT) and intraoval precipitin (IOP) reaction for the diagnosis of schistosomiasis japonica. The liver tissue of an infected mouse with the Philippine strain of Schistosoma japonicum was prepared according to the routine process for histopathological sectioning. Specific fluorescence was detected in the space between the vitelline membrane and the surface of miracidia in the egg, and it disappeared with periodic acid treatment even though the space had been strongly PAS stained. The formalin fixed tissue section also revealed an IOP reaction in the area where the specific fluorescence was detected. These results indicated that the antigen response to the IFAT and IOP tests corresponded to the responses of heat stable substances such as polysaccharides or glycoproteins. We suggested that formalin fixed sections are more practicable in IFAT and IOP tests than are cryostat processed tissue sections and lyophilized or fresh whole eggs for the diagnosis of schistosomiasis or other parasitic infections.

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