界面活性剤
小澤, 真一郎;高橋, 裕一郎
Permalink : http://hdl.handle.net/2115/39174
Abstract
界面活性剤は光合成タンパク質の生化学的解析によく用いられる.電気泳動法による分析では,タンパク質の可溶化,変性,電荷の付与などに,膜タンパク質の精製・分析では,疎水性タンパク質の可溶化に使われる.本章では,一般的に使われる界面活性剤について紹介する.
Detergents are frequently used for biochemical analyses of photosynthetic proteins. For polyacrylamide gel electrophoresis, detergents are used to solubilize and/or denature proteins. For purification of membrane proteins, detergents are also required for solubilization of water-insoluble proteins. In this chapter, detergents that are frequently used in protein biochemistry are introduced.
Detergents are frequently used for biochemical analyses of photosynthetic proteins. For polyacrylamide gel electrophoresis, detergents are used to solubilize and/or denature proteins. For purification of membrane proteins, detergents are also required for solubilization of water-insoluble proteins. In this chapter, detergents that are frequently used in protein biochemistry are introduced.
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