Japanese Journal of Veterinary Research;Volume 36, Number 2

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LIGHT AND ELECTRON MICROSCOPIC STUDIES ON CHICKEN INTESTINAL GLOBULE LEUCOCYTES

KITAGAWA, Hiroshi;HASHIMOTO, Yoshiharu;KON, Yasuhiro;KUDO, Norio

Permalink : http://hdl.handle.net/2115/3087
JaLCDOI : 10.14943/jjvr.36.2.83
KEYWORDS : chicken intestine;emiocytosis;globule leucocytes;natural killer cells;ultrastructure

Abstract

Chicken intestinal globule leucocytes (GL) and various intraepithelial migrating cells were light and electron microscopically investigated. The morphological characteristics of GL were consistent with those of intraepithelial lymphocytes, except for the high cytoplasmic/nucleus ratio, the well-developed Golgi apparatus and the cytoplasmic granules. GL could be light and electron microscopically distinguished from mast cells, plasma cells (Russel body cells), and other migrating cells. Plasma cells and macrophages were located in the epithelium covering the large lymphatic nodules, but not in the ordinary columnar epithelium. However, GL and lymphocytes populated both epithelia. The cellular traffic of GL and lymphocytes across the basement membrane of the mucous epithelium and the penetration of lymphocytes into the intestinal lumen were often found. The granules of GL, which located in the area neighboring Golgi complexes, were classified into two types ; type I consisted only of amorphous matrix (AM), and type II of a marginal zone of fine reticular materials (FRM) in addition to the AM. As the FRM zone became wider, the AM became more irregular in shape. Crystalline or lamellar inclusions were recognized in both AM and FRM. The directions of the crystalline arrays in the AM were all slightly different. Fusion of the granules was rarely found. It was noted that the rough-surfaced endoplasmic reticulum rarely connected with the type II granules. The FRM of the type II granules was released to the extracellular space by emiocytosis. The release of the AM was prevented by the cytoplasmic processes or folds at the outlets of the granules. The secretory products filled the intercellular space between GL and the neighboring epithelial cells. These results suggested a morphological similarity between GL and natural killer cells, and clarified the formation processes and the fate of the granules of GL.

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