Japanese Journal of Veterinary Research;Volume 8, Number 1-4

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IMMUNOLOGICAL STUDIES ON THE INFECTIOUS CANINE HEPATITIS VIRUS : II. HEMAGGLUTININ

SHIMIZU, Yukio;KUNISHIGE, Teruo;HIRATO, Katsushichi

Permalink : http://hdl.handle.net/2115/4685
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Abstract

Experiments on the hemagglutination reaction by ICH virus were performed. The results are summarized as follows : 1) Two strains of ICH virus, "MATSUDA" and "Woc-4", agglutinated erythrocytes of human and guinea pigs under certain conditions. 2) HAT was affected by many environmental factors such as pH and electrolyte concentration of the reaction mixture, incubation temperature, concentration of erythrocytes and so on. Optimum conditions for the reaction were determined in respect to these factors. 3) Heat stability of infecting virus particle, CFA and hemagglutinin was studied and the relationship among these three components was discussed on the basis of the results of adsorption and elution experiments. 4) Normal dog sera were found to contain different amounts of normal inhibitor and hemagglutinin. Some methods to remove these substances are now under investigation. The authors wish to express their gratitude to Dr. OCHI, Tokyo University, for his helpful support in providing the strain of ICH virus.

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