Japanese Journal of Veterinary Research;Volume 67 Number 2

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Serum amyloid A concentrations in cats measured using a newly developed feline-specific latex agglutination immunoassay

Ishioka, Katsumi;Hayakawa, Noriyuki

Permalink : http://hdl.handle.net/2115/74795
JaLCDOI : 10.14943/jjvr.67.2.145
KEYWORDS : cats;latex agglutination immunoassay;Serum amyloid A

Abstract

Serum amyloid A (SAA) is an acute phase protein that is utilized as an inflammatory marker for cats. Since no feline-specific reagents are available in clinical laboratories to measure SAA, human SAA reagents have been used in many veterinary laboratories. In the present study, we developed a new assay system to measure feline SAA using a latex agglutination immunoassay with an anti-feline SAA antibody that can be run on autoanalyzers. This new assay had increased sensitivity for feline serum SAA compared to the previous human assay. The standard SAA value in healthy cats was less than 5.22 μg/mL. Bilirubin, hemoglobin, and chylomicron did not interfere with the assay. Serum SAA concentrations in 17 patient cats with inflammation or tumors were approximately 30-fold higher than in normal cats (P < 0.0001). This is the first report on feline SAA concentrations measured using a laboratory-use specific assay, which would benefit feline medicine.

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