Japanese Journal of Veterinary Research;Volume 2, Number 4

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PRELIMINARY INVESTIGATIONS ON COMPLEMENT-FIXING ANTIGENS IN BOVINE TUBERCULOSIS

SHIMIZU, Kiheiji

Permalink : http://hdl.handle.net/2115/1663
JaLCDOI : 10.14943/jjvr.2.4.157

Abstract

The principal subject discussed in the present paper is the antigenicities of several kinds of antigens for the C. F. T. in bovine tuberculosis. 1. The antigenicities of next-mentioned human and bovine type of antigens were examined ; Boiling supernatant, bacterial carbohydrate, bacterial protein, ether-extracted, ether-alcohol-extracted lipoids which were prepared from the dried bacilli preliminarily inactivated by temperature of 70℃ and 100℃, and tuberculin. 2. The boiling supernatant antigens from the human type bacilli by low temperature inactivation and from the bovine type bacilli inactivated by both low and high temperature, equally indicated very good antigenicity and specificity. 3. Bacterial proteins possess considerably good antigenicity, however, their reaction ranges was not so wide but that they easily lost their antigenicity by comparative low dilutions. 4. Tuberculin also indicated excellent antigenicity but the nonspecific reactions were sometimes recognized. 5. Ether-extracted lipoid antigens are not recommendable ones on account of their weak antigenicity, especially in the one from bovine type, and on account of non-specific reactions. 6. Bacterial carbohydrate antigens do not have any antigenicity at all.

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