Japanese Journal of Veterinary Research;Volume 19, Number 1-2

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FLUORESCENT ANTIBODY TECHNIQUE IN CULTURED CELLS INFECTED WITH PORCINE ENTEROVIRUSES

WATANABE, Hiroshi

Permalink : http://hdl.handle.net/2115/1968
JaLCDOI : 10.14943/jjvr.19.1-2.1

Abstract

The indirect method of the fluorescent antibody (FA) technique was applied to observe primary porcine kidney cultured cells infected with porcine enterovirusess (V 76 and Kr 69 TC strains). Halo-like specific fluorescence was present in one part of the cytoplasms adjoined to the nuclei of the infected cells and rather large fluorescent granules were also scattered there 5 hours after inoculation (HAI), the first observation time. In the following time, the fluorescent granules tended to become smaller and to aggregate densely in the cytoplasms surrounding the nuclei of the cells. Twenty to 25 HAI, a cytopathic effect (CPE) consisting of shrinking and rounding of the fluorescent cells became obvious. The cellular and nuclear membranes became indistinct and dust-like fluorescenet granules filled the cytoplasms and the nuclei. The most suitable observation time in the diagnostic tests was confirmed to be from 15 to 20 HAI.

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