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Volume 19, Number 1-2 >

FLUORESCENT ANTIBODY TECHNIQUE IN CULTURED CELLS INFECTED WITH PORCINE ENTEROVIRUSES

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Please use this identifier to cite or link to this item:http://doi.org/10.14943/jjvr.19.1-2.1

Title: FLUORESCENT ANTIBODY TECHNIQUE IN CULTURED CELLS INFECTED WITH PORCINE ENTEROVIRUSES
Authors: WATANABE, Hiroshi Browse this author
Issue Date: Jun-1971
Publisher: The Graduate School of Veterinary Medicine
Journal Title: Japanese Journal of Veterinary Research
Volume: 19
Issue: 1-2
Start Page: 1
End Page: 5
Abstract: The indirect method of the fluorescent antibody (FA) technique was applied to observe primary porcine kidney cultured cells infected with porcine enterovirusess (V 76 and Kr 69 TC strains). Halo-like specific fluorescence was present in one part of the cytoplasms adjoined to the nuclei of the infected cells and rather large fluorescent granules were also scattered there 5 hours after inoculation (HAI), the first observation time. In the following time, the fluorescent granules tended to become smaller and to aggregate densely in the cytoplasms surrounding the nuclei of the cells. Twenty to 25 HAI, a cytopathic effect (CPE) consisting of shrinking and rounding of the fluorescent cells became obvious. The cellular and nuclear membranes became indistinct and dust-like fluorescenet granules filled the cytoplasms and the nuclei. The most suitable observation time in the diagnostic tests was confirmed to be from 15 to 20 HAI.
Type: bulletin (article)
URI: http://hdl.handle.net/2115/1968
Appears in Collections:Japanese Journal of Veterinary Research > Volume 19, Number 1-2

Submitter: 獣医学部図書室

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