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Japanese Journal of Veterinary Research >
Volume 19, Number 1-2 >

FLUORESCENT ANTIBODY TECHNIQUE IN CULTURED CELLS INFECTED WITH PORCINE ENTEROVIRUSES

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この文献には次のDOIがあります:http://doi.org/10.14943/jjvr.19.1-2.1

タイトル: FLUORESCENT ANTIBODY TECHNIQUE IN CULTURED CELLS INFECTED WITH PORCINE ENTEROVIRUSES
著者: WATANABE, Hiroshi 著作を一覧する
発行日: 1971年 6月
出版者: The Graduate School of Veterinary Medicine
誌名: Japanese Journal of Veterinary Research
巻: 19
号: 1-2
開始ページ: 1
終了ページ: 5
抄録: The indirect method of the fluorescent antibody (FA) technique was applied to observe primary porcine kidney cultured cells infected with porcine enterovirusess (V 76 and Kr 69 TC strains). Halo-like specific fluorescence was present in one part of the cytoplasms adjoined to the nuclei of the infected cells and rather large fluorescent granules were also scattered there 5 hours after inoculation (HAI), the first observation time. In the following time, the fluorescent granules tended to become smaller and to aggregate densely in the cytoplasms surrounding the nuclei of the cells. Twenty to 25 HAI, a cytopathic effect (CPE) consisting of shrinking and rounding of the fluorescent cells became obvious. The cellular and nuclear membranes became indistinct and dust-like fluorescenet granules filled the cytoplasms and the nuclei. The most suitable observation time in the diagnostic tests was confirmed to be from 15 to 20 HAI.
資料タイプ: bulletin
URI: http://hdl.handle.net/2115/1968
出現コレクション:Volume 19, Number 1-2

 

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