Japanese Journal of Veterinary Research;Volume 42, Number 2

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A NEUTRAL SUGAR IS RESPONSIBLE FOR SEROVAR SPECIFICITY OF THE ANTIGENIC DETERMINANT OF LEPTOSPIRA INTERROGANS SEROVAR CANICOLA

KASHIWASE, Hiroto;ONO, Etsuro;YANAGAWA, Ryo;SHIMIZU, Yukio;KIDA, Hiroshi

Permalink : http://hdl.handle.net/2115/2471
JaLCDOI : 10.14943/jjvr.42.2.103
KEYWORDS : Leptospira;glycolipid;antigen;determinant;sugar

Abstract

To provide information on the chemical structures of antigenic determinants of leptospira, glycolipids of Leptospira interrogans serovar canicola strain Hond Utrecht IV (Ut-IV) and its antigenic variant selected in the presence of a serovar-specific monoclonal antibody were compared physicochemically. Gasliquid chromatography-mass spectrometry analysis revealed that the glycolipid of Ut-IV contained 6 neutral sugar species ; rhamnose, mannose, galactose, glucose, and unknown sugars III and IV, in addition to unknown sugars I and II that had been previously reported. On the other hand, the glycolipid of the variant lacked unknown sugar III, suggesting that this sugar is responsible for the serovar-specific antigenic determinant.

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