Japanese Journal of Veterinary Research;Volume 50, Number 4


Passive immunization with monoclonal antibodies : effects on Haemaphysalis longicornis tick infestation of BALB/c mice

Nakajima, Mie;Yanase, Haruko;Iwanaga, Toshihiko;Kodama, Michi;Ohashi, Kazuhiko;Onuma, Misao

Permalink : http://hdl.handle.net/2115/2963
JaLCDOI : 10.14943/jjvr.50.4.157
KEYWORDS : Haemaphysalis longicornis;midgut;monoclonal antibodies;passive immunization


Tick vaccine development plays an important role in current tick control strategies. Previously, we have produced three different isotypes of monoclonal antibodies (mAbs) which recognized a midgut protein of adult Haemaphysalis longicornis. These mAbs, typed as IgG1, 2a, and 2b, reacted with a 76 kDa surface protein of midgut cells. We speculated that the 76 kDa protein may be an unknown antigen for a tick vaccine and the three mAbs may work as probes to clone the protein. In this study, to test whether these three isotypes have anti-tick effects and if so which works more effectively, we conducted passive immunization in BALB/c mice with each of the mAbs, and infested the mice with adult ticks. All isotypes significantly reduced the number of hatched larvae, compared to controls, however, no differences in the magnitude of the reduction were observed among the three.