Japanese Journal of Veterinary Research;Volume 61 Number 1&2

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Inhibition of influenza A virus infection by Galectin-9

Hattori, Tomoe;Arikawa, Tomohiro;Fujioka, Yoichiro;Maruyama, Junki;Nakayama, Yousuke;Ohba, Yusuke;Niki, Toshiro;Miyazaki, Tadaaki;Hirashima, Mitsuomi;Kida, Hiroshi

Permalink : http://hdl.handle.net/2115/52827
JaLCDOI : 10.14943/jjvr.61.1-2.5
KEYWORDS : Gal-9;influenza A virus;inhibition of viral growth

Abstract

Galectin-9 (Gal-9) inhibited the infection of H1N1, H3N2 and H5N1 influenza A viruses in vitro and in vivo. Fifty percent effective doses (ED50) of Gal-9 were 0.1-0.5 M depending on virus strains in the plaque reduction assay. Gal-9 but not Gal-1 bound to the virus particles of A/Puerto Rico/8/34 (H1N1) (PR/8), resulting in inhibition of virus attachment to the host cells. Lactose but not sucrose inhibited the binding of Gal-9 to the viruses. Endogenous Gal-9 expression was detected and increased with the course of infection with influenza A viruses in mice. Fifty percent of Gal-9-transgenic mice survived after the challenge with PR/8, while all of the wild-type mice died. Gal-9 treatment of mice affected diminishing influenza virus replication in the lungs, body weight loss and the expression level of inflammatory cytokines. Combined administration of Gal-9 and oseltamivir was more effective than the use of single compound in mouse model. The present results indicate that Gal-9 is a candidate compound for influenza A virus infection therapy.

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