Japanese Journal of Veterinary Research;Volume 61 Number 4

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In vitro maturation system for individual culture of bovine oocytes using micro-volume multi-well plate

Nagano, Masashi;Kang, Sung-Sik;Koyama, Keisuke;Huang, Weiping;Yanagawa, Yojiro;Takahashi, Yoshiyuki

Permalink : http://hdl.handle.net/2115/53709
JaLCDOI : 10.14943/jjvr.61.4.149
KEYWORDS : IVM;micro-well;oocyte

Abstract

As a preliminary study for the development of individual in vitro maturation (IVM) culture of bovine oocytes, a multi-well (MW) plate was used. Maturation, fertilization and development to blastocysts were examined and compared with those of IVM oocytes cultured in 50-µl droplets in groups and in 10-µl droplets individually. The maturation rates were similar in all experimental groups. Normal fertilization rates in MW and 50-µl droplets were similar, but lower in 10-µl droplets (p < 0.01). The blastocyst rate in 10-µl droplets tended to be lower than those in MW (p = 0.15) and 50-µl droplets (p = 0.19). These results indicate that an IVM system using MW supports the acquisition of developmental competence by bovine oocytes the same as conventional group IVM culture.

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