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Volume 61 Number 4 >

In vitro maturation system for individual culture of bovine oocytes using micro-volume multi-well plate

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Please use this identifier to cite or link to this item:http://doi.org/10.14943/jjvr.61.4.149

Title: In vitro maturation system for individual culture of bovine oocytes using micro-volume multi-well plate
Authors: Nagano, Masashi Browse this author
Kang, Sung-Sik Browse this author
Koyama, Keisuke Browse this author
Huang, Weiping Browse this author
Yanagawa, Yojiro Browse this author
Takahashi, Yoshiyuki Browse this author
Keywords: IVM
micro-well
oocyte
Issue Date: Nov-2013
Publisher: Graduate School of Veterinary Medicine, Hokkaido University
Journal Title: Japanese Journal of Veterinary Research
Volume: 61
Issue: 4
Start Page: 149
End Page: 154
Abstract: As a preliminary study for the development of individual in vitro maturation (IVM) culture of bovine oocytes, a multi-well (MW) plate was used. Maturation, fertilization and development to blastocysts were examined and compared with those of IVM oocytes cultured in 50-µl droplets in groups and in 10-µl droplets individually. The maturation rates were similar in all experimental groups. Normal fertilization rates in MW and 50-µl droplets were similar, but lower in 10-µl droplets (p < 0.01). The blastocyst rate in 10-µl droplets tended to be lower than those in MW (p = 0.15) and 50-µl droplets (p = 0.19). These results indicate that an IVM system using MW supports the acquisition of developmental competence by bovine oocytes the same as conventional group IVM culture.
Type: bulletin (article)
URI: http://hdl.handle.net/2115/53709
Appears in Collections:Japanese Journal of Veterinary Research > Volume 61 Number 4

Submitter: 獣医学部図書室

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