Japanese Journal of Veterinary Research;Volume 64 Supplement 2


Prevalence, antibiogram, molecular characterization and reduction trial of Salmonella typhimurium isolated from different fish species

Bayomi, Rasha M. El;Darwish, Wageh S.;El-Moaty, Amany M. Abd;Gad, Tamer M.

Permalink : http://hdl.handle.net/2115/62007
KEYWORDS : Antibiotic resistance;fish;Salmonella;virulent genes;liquid smoke


Salmonella is a major cause of food-borne outbreaks and infections in many countries worldwide. The present work aimed to investigate the prevalence, antibiotic sensitivity and detection of virulence-associated genes in Salmonella typhimurium isolated from fish samples (tilapia, mullet and catfish) collected from Zagazig city markets, Egypt. Salmonella typhimurium was isolated only from tilapia with a percentage of 13.3%. Antibiotic susceptibility testing of Salmonella typhimurium revealed marked susceptibility to ofloxacin. Two different virulence genes (ssaP and pipB) were expressed in the isolated Salmonella typhimurium. Dipping of tilapia in liquid smoke 5% for 30 min reduced Salmonella typhimurium count by 40%. In conclusion, our results confirm contamination of tilapia by Salmonella typhimurium. Dipping of fish in liquid smoke is an efficient strategy in reducing Salmonella typhimurium load in fish.