Japanese Journal of Veterinary Research;Volume 68 Number 2

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First molecular identification of Trypanosoma evansi from cattle in Syria

Megasari, Marsela;Kyoko, Hayashida;Alaa, Terkawi;Xuenan, Xuan;Chihiro, Sugimoto;Junya, Yamagishi

Permalink : http://hdl.handle.net/2115/78622
JaLCDOI : 10.14943/jjvr.68.2.117
KEYWORDS : Epidemiology;ITS1-PCR;Syria;Trypanosoma evansi

Abstract

Trypanosoma evansi, the “surra” disease-causing agent, is a blood protozoan parasite that infects a wide range of mammalian species within an unlimited geographical region. It causes anemia, weight loss, and even death of the infected livestock that heavily affect animal husbandry. However, the full epidemiological information of T. evansi is lacking, especially in developing countries, and the risk of the disease is largely underestimated. In this study, 207 samples of blood DNA collected from Holstein Friesian crossbred cattle in the central region of Syria in May 2010 were screened for T. evansi, aiming to determine the prevalence of the parasite. T. evansi was screened by PCR targeting the internal transcribed spacer (ITS) 1 region, and 27 samples were found positive out of 207 (13%), which is relatively high considering that no clinical symptoms were observed. The ITS1 amplicons were later subjected to RoTat1.2-PCR for detection of T. evansi type A. This is the first report of molecular detection of T. evansi in Syria. Our study suggests that advanced investigations in cattle and other domestic animals are necessary in Syria.

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