Mechanism of the toxicity induced by natural humic acid on human vascular endothelial cells

Kihara, Yusuke; Yustiawati; Tanaka, Masato; Gumiri, Sulmin; Ardianor; Hosokawa, Toshiyuki; Tanaka, Shunitz; Saito, Takeshi; Kurasaki, Masaaki

Mechanism of the toxicity induced by natural humic acid on human vascular endothelial cells

Kihara, Yusuke; Yustiawati; Tanaka, Masato et al.

Environmental Toxicology, 2014, 29(8), 916-925

Permalink : https://hdl.handle.net/2115/53585

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論文情報

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アクセス権	open access
URI	https://hdl.handle.net/2115/53585
タイトル	Mechanism of the toxicity induced by natural humic acid on human vascular endothelial cells
著者	Kihara, Yusuke ; Yustiawati ; Tanaka, Masato ; Gumiri, Sulmin ; Ardianor ; Hosokawa, Toshiyuki ; Tanaka, Shunitz ; Saito, Takeshi ; Kurasaki, Masaaki
著者名 Kihara, Yusuke
著者名 Yustiawati
著者名 Tanaka, Masato
著者名 Gumiri, Sulmin
著者名 Ardianor
e-Rad_Researcher 00157025 著者名 Hosokawa, Toshiyuki
e-Rad_Researcher 30142194 著者名 Tanaka, Shunitz
e-Rad_Researcher 40153811 著者名 Saito, Takeshi
e-Rad_Researcher 80161727 著者名 Kurasaki, Masaaki
言語	英語
キーワード	humic acid
	tropical peatland
	apoptosis
	endothelial cell
	oxidative stress
発行日	2014-08
出版者	Wiley
収録物名	Environmental Toxicology
巻(号)	29(8)
ページ	916-925
抄録	Humic acid (HA), a group of high-molecular weight organic compounds characterized by an ability to bind heavy metals, is normally found in natural water. Although the impairment of vascular endothelial cells in the presence of humic substances has been reported to be involved in some diseases, the mechanisms responsible for this involvement remain unclear. In this study, we examined the cytotoxicity of HA obtained from peatland in Central Kalimantan, Indonesia, to human vascular endothelial cells, as well as the mechanisms behind these effects. It was found that 50 mg/L HA showed cytotoxicity, which we considered to be mediated by apoptosis through the mitochondrial pathway because of an increase in the expression of caspases 6 and 9 in response to HA administration. In addition, this cytotoxicity was enhanced when cells in this experimental system were exposed to oxidative stress, while it was decreased by the addition of vitamin C. Thus, we conclude that the apoptosis induced by HA depends upon oxidative stress. Furthermore, an iron chelator, DFO, showed a tendency to decrease HA-induced cytotoxicity, suggesting that iron may potentially mediate HA-induced oxidative stress. In conclusion, long-term consumption of HA-rich water obtained from our study area may cause damage to endothelial cells and subsequent chronic health problems. © 2012 Wiley Periodicals, Inc. Environ Toxicol, 2012.
資源タイプ	学術雑誌論文
出版タイプ	Submitted Manuscript Under Review
権利情報	This is the pre-peer reviewed version of the following article: Kihara, Y., Yustiawati, , Tanaka, M., Gumiri, S., Ardianor, , Hosokawa, T., Tanaka, S., Saito, T. and Kurasaki, M. (2014), Mechanism of the toxicity induced by natural humic acid on human vascular endothelial cells. Environ. Toxicol, 29: 916-925., which has been published in final form at https://doi.org/10.1002/tox.21819.
PISSN	1520-4081
NCID	AA11354769
関連情報	PMID 23042718
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1002/tox.21819