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Volume 52, Number 1 >

Evidence for bovine immunodeficiency virus infection in cattle in Zambia

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Title: Evidence for bovine immunodeficiency virus infection in cattle in Zambia
Authors: Meas, Sothey Browse this author
Nakayama, Megumi Browse this author
Usui, Tstsufumi Browse this author
Nakazato, Yoshikazu Browse this author
Yasuda, Jun Browse this author
Ohashi, Kazuhiro Browse this author
Onuma, Misao Browse this author
Keywords: bovine immunodeficiency virus
bovine leukemia virus
molecular evidence
Issue Date: May-2004
Publisher: The Graduate School of Veterinary Medicine, Hokkaido University
Journal Title: Japanese Journal of Veterinary Research
Volume: 52
Issue: 1
Start Page: 3
End Page: 8
Abstract: We report herein on the first evidence for presence of bovine immunodeficiency virus (BIV) in Zambia. Serological surveillance of BIV and bovine leukemia virus (BLV) was conducted in traditional cattle herds in Zambia. Out of a total of 262 sera analyzed, 11.4% were found positive for anti-BIV p26 antibodies as determined by Western blot analysis, while 5.0% were positive for anti-BLV gp51 antibodies as detected by immunodiffusion test. Peripheral blood mononuclear cells from BIV seropositive cattle were found to have BIV-provirus DNA, as detected by nested polymerase chain reaction. A nucleotide sequence corresponding to a 298 bp fragment of BIV pol gene wea also analyzed. Amino acid sequences of these Zambian pol gene products showed 98.0 to 100% homology to the American strain BIV R29, 97.0 to 99.0% to Japanese BIV isolates, and divergence ranged from 0.0 to 2.0% among Zambian BIV isolates.
Type: bulletin (article)
Appears in Collections:Japanese Journal of Veterinary Research > Volume 52, Number 1

Submitter: 獣医学部図書室

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