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Volume 52, Number 1 >

Evidence for bovine immunodeficiency virus infection in cattle in Zambia

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タイトル: Evidence for bovine immunodeficiency virus infection in cattle in Zambia
著者: Meas, Sothey 著作を一覧する
Nakayama, Megumi 著作を一覧する
Usui, Tstsufumi 著作を一覧する
Nakazato, Yoshikazu 著作を一覧する
Yasuda, Jun 著作を一覧する
Ohashi, Kazuhiro 著作を一覧する
Onuma, Misao 著作を一覧する
キーワード: bovine immunodeficiency virus
bovine leukemia virus
molecular evidence
発行日: 2004年 5月
出版者: The Graduate School of Veterinary Medicine, Hokkaido University
誌名: Japanese Journal of Veterinary Research
巻: 52
号: 1
開始ページ: 3
終了ページ: 8
抄録: We report herein on the first evidence for presence of bovine immunodeficiency virus (BIV) in Zambia. Serological surveillance of BIV and bovine leukemia virus (BLV) was conducted in traditional cattle herds in Zambia. Out of a total of 262 sera analyzed, 11.4% were found positive for anti-BIV p26 antibodies as determined by Western blot analysis, while 5.0% were positive for anti-BLV gp51 antibodies as detected by immunodiffusion test. Peripheral blood mononuclear cells from BIV seropositive cattle were found to have BIV-provirus DNA, as detected by nested polymerase chain reaction. A nucleotide sequence corresponding to a 298 bp fragment of BIV pol gene wea also analyzed. Amino acid sequences of these Zambian pol gene products showed 98.0 to 100% homology to the American strain BIV R29, 97.0 to 99.0% to Japanese BIV isolates, and divergence ranged from 0.0 to 2.0% among Zambian BIV isolates.
資料タイプ: bulletin
出現コレクション:Volume 52, Number 1


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