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The rpoH gene encoding heat shock sigma factor sigma(32) of psychrophilic bacterium Colwellia maris

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タイトル: The rpoH gene encoding heat shock sigma factor sigma(32) of psychrophilic bacterium Colwellia maris
著者: Yamauchi, Seiji 著作を一覧する
Okuyama, Hidetoshi 著作を一覧する
Nishiyama, Yoshitaka 著作を一覧する
Hayashi, Hidenori 著作を一覧する
キーワード: Psychrophilic bacterium
Colwellia maris
rpoH gene
sigma(32)
Heat shock proteins
発行日: 2006年 4月
出版者: Springer Japan
誌名: Extremophiles
巻: 10
号: 2
開始ページ: 149
終了ページ: 158
出版社 DOI: 10.1007/s00792-005-0485-9
抄録: The rpoH gene encoding a heat shock sigma factor, sigma(32), was cloned from the psychrophilic bacterium Colwellia maris. The deduced amino acid sequence of sigma(32) from C. maris is more than 60% homologous to that of sigma(32) from mesophilic bacteria. The RpoH box, a 9-amino-acid sequence region (QRKLFFNLR) specific to sigma(32), and two downstream box sequences complementary to a part of 16S rRNA were identified. Primer extension analysis showed that the C. maris rpoH is expressed from only one sigma(70)-type promoter. Northern blot analysis showed that the level of rpoH mRNA was clearly increased at 20°C, a temperature that induces heat shock in this organism. In the presence of an inhibitor of transcriptional initiation, the degradation of rpoH mRNA was much slower at 20°C than at 10°C. Thus, increased stability of the rpoH mRNA might be responsible for the rpoH mRNA accumulation. The predicted secondary structure of the 5'-region of C. maris rpoH mRNA was different from the conserved patterns reported for most mesophilic bacteria, and the base pairing of the downstream boxes appeared to be less stable than that of Escherichia coli rpoH mRNA. Thus, essential features that ensure the HSP expression at a relatively low temperature are embedded in the rpoH gene of psychrophiles.
Rights: The original publication is available at www.springerlink.com
Relation (URI): http://www.springerlink.com
資料タイプ: article
URI: http://hdl.handle.net/2115/10092
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 奥山 英登志

 

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