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Volume 52, Number 3 >

Inactivation of SARS coronavirus by means of povidone-iodine, physical conditions, and chemical reagents

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Please use this identifier to cite or link to this item:http://doi.org/10.14943/jjvr.52.3.105

Title: Inactivation of SARS coronavirus by means of povidone-iodine, physical conditions, and chemical reagents
Authors: Kariwa, Hiroaki Browse this author →KAKEN DB
Fujii, Nobuhiro Browse this author
Takashima, Ikuo Browse this author
Keywords: coronavirus
disinfection
inactivation
infection control
povidone-iodine
SARS
Severe acute respiratory syndrome
Issue Date: Nov-2004
Publisher: The Graduate School of Veterinary Medicine, Hokkaido University
Journal Title: Japanese Journal of Veterinary Research
Volume: 52
Issue: 3
Start Page: 105
End Page: 112
Abstract: products, a number of other chemical agents, and various physical conditions were evaluated for their ability to inactivate the severe acute respiratory syndrome coronavirus (SARS-CoV). Treatment of SARS-CoV with PVP-I products for 2 min reduced the virus infectivity from 1.17 x 10⁶ TCID₅₀/ml to below the detectable level. The efficacy of 70% ethanol was equivalent to that of PVP-I products. Fixation of SARS-CoV-infected Vero E6 cells with a fixative including formalin, glutaraldehyde, methanol, and acetone for 5 min or longer eliminated all infectivity. Heating the virus at 56℃ for 5 min dramatically reduced the infectivity of the virus from 2.6 x 10⁷ to 40 TCID₅₀/ml, whereas heating the virus for 60 min or longer eliminated all infectivity. Irration with ultraviolet light at 134μQ/cm² for 15 min reduced the infectivity from 3.8 x 10⁷ to 180 TCID₅₀/ml; however, prolonged irradiation (60 min) failed to eliminate the remaining virus, leaving 18.8 TCID₅₀/ml. We beleave that these finding will be useful for the implementation of infection control measures against SARS, and for the establishment of effective guidlines for the preventation of SARS outbreaks.
Type: bulletin (article)
URI: http://hdl.handle.net/2115/10516
Appears in Collections:Japanese Journal of Veterinary Research > Volume 52, Number 3

Submitter: 獣医学部図書室

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