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A novel preparation of microcast for wood micromorphology using polydimethylsiloxane without digesting cell wall

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Title: A novel preparation of microcast for wood micromorphology using polydimethylsiloxane without digesting cell wall
Authors: Uraki, Yasumitsu1 Browse this author →KAKEN DB
Nemoto, Junji Browse this author
Sano, Yuzou Browse this author →KAKEN DB
Authors(alt): 浦木, 康光1
Keywords: microcast
silicon elastomer
PDMS
cell wall sculpture
Issue Date: Apr-2006
Publisher: Springer
Journal Title: Journal of Wood Science
Volume: 52
Issue: 2
Start Page: 163
End Page: 166
Publisher DOI: 10.1007/s10086-005-0740-9
Abstract: We developed a novel method for preparation of microcasts of wood with silicone elastomer (polydimethylsiloxane; PDMS). PDMS was so flexible and elastic that it was possible to isolate the microcasts by simply pulling them out of the mold without digesting the cell wall after the resin was cured for 2 days at room temperature. The casts of some cell wall sculptures, such as spiral thickenings and bordered pits, had high fidelity. By contrast, the casts of distinctly bordered pits and tails of vessel elements were often deformed or broken. Bars of scalariform perforation plates were always torn and remained in the resin casts. The microcast preparation using PDMS is useful for easy investigation of cell wall sculptures. It might be also useful for microfractography of bars of scalariform perforation plates.
Rights: The original publication is available at www.springerlink.com
Type: article (author version)
URI: http://hdl.handle.net/2115/10520
Appears in Collections:農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 浦木 康光

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