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Volume 51, Number 3-4 >

Characterization of the Chicken PKR : Polymorphism of the gene and Antiviral Activity against Vesicular Stomatitis Virus

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Title: Characterization of the Chicken PKR : Polymorphism of the gene and Antiviral Activity against Vesicular Stomatitis Virus
Authors: Ko, Jae-Hong Browse this author
Asano, Atsushi Browse this author
Kon, Yasuhiro Browse this author →KAKEN DB
Watanabe, Tomomasa Browse this author
Agui, Takashi Browse this author →KAKEN DB
Keywords: Chiken
Vesicular stomatitis virus
Issue Date: Feb-2004
Publisher: The Graduate School of Veterinary Medicine, Hokkaido University
Journal Title: Japanese Journal of Veterinary Research
Volume: 51
Issue: 3-4
Start Page: 123
End Page: 133
Abstract: The double-stranded RNA-dependent protein kinase (PKR) is induced in mouse and human cells on treatment with interferon.In this study, we have cloned and determined the nucleotide sequences of chicken PKR cDNA in various chicken breeds. Chicken PKR was a 550-amino-acid protein as deduced from the cDNA open reading frame (ORF), and there were specific domains (two double-stranded RNA binding domains (DRBDs) and numerous kinase subdomains) characterized in RNA binding proteins and kinase families. Furthermore, it was suggested that chicken PKR was polymorphic. Transfected cell clones expressing chicken PKR mRNA were demonstrated to confer antiviral responses to vesicular stomatitis virus, except for Koshamo type-3 (KS-3). KS-3 PKR, which has an amino acid substitution at position 507 (Arg to Gln), showed amphibious antiviral responses. This specific amino acid substitution was considered to determine the antiviral function of chicken PKR in addition to essential domains as DRBDs and kinase subdomains.
Type: bulletin (article)
Appears in Collections:Japanese Journal of Veterinary Research > Volume 51, Number 3-4

Submitter: 獣医学部図書室

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