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Hypoxia enhances the expression of autocrine motility factor and the motility of human pancreatic cancer cells

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タイトル: Hypoxia enhances the expression of autocrine motility factor and the motility of human pancreatic cancer cells
著者: Niizeki, Hiroto 著作を一覧する
Kobayashi, Masanobu 著作を一覧する
Horiuchi, Iori 著作を一覧する
Akakura, Nobuaki 著作を一覧する
Chen, Jian 著作を一覧する
Wang, Jingxin 著作を一覧する
Hamada, Jun-ichi 著作を一覧する
Seth, Prem 著作を一覧する
Katoh, Hiroyuki 著作を一覧する
Watanabe, Hideomi 著作を一覧する
Raz, Avraham 著作を一覧する
Hosokawa, Masuo 著作を一覧する
キーワード: hypoxia
AMF
motility
HIF-1
metastasis
発行日: 2002年 6月17日
出版者: Nature Publishing Group
誌名: British Journal of Cancer
巻: 86
号: 12
開始ページ: 1914
終了ページ: 1919
出版社 DOI: 10.1038/sj.bjc.6600331
抄録: The incidence of distant metastases is higher in the tumors with low oxygen pressure than in those with high oxygen pressure. It is well known that hypoxia induces the transcription of various genes involved in angiogenesis and anaerobic metabolism necessary for the growth of tumor cells in vivo, suggesting that hypoxia may also induce the transcription of metastasis-associated genes. We sought to identify the metastasis-associated genes differentially expressed in tumor cells under hypoxic conditions with the use of a DNA microarray system. We found that hypoxia enhanced the expression of autocrine motility factor (AMF) mRNA in various cancer cells and also enhanced the random motility of pancreatic cancer cells. AMF inhibitors abrogated the increase of motility under hypoxic conditions. In order to explore the roles of hypoxia-inducible factor-1α (HIF-1α), we established HIF-1α-transfectants and dominant negative HIF-1α-transfectants. Transfection with HIF-1α and dominant-negative HIF-1α enhanced and suppressed the expression of AMF/PHI/NL mRNA and the random motility, respectively. These results suggest that hypoxia may promote the metastatic potential of cancer cells through the enhanced AMF/PHI/NL mRNA expression and that the disruption of the HIF-1 pathway may be an effective treatment for metastasis.
資料タイプ: article (author version)
URI: http://hdl.handle.net/2115/11317
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 小林 正伸

 

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