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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences >
Peer-reviewed Journal Articles, etc >
Selective Foam Separation of Binary Protein Solution
| Title: | Selective Foam Separation of Binary Protein Solution |
| Other Titles: | Selective Foam Separation of Binary Protein Solution by SDS Complexation Method. |
| Authors: | Suzuki, Akira Browse this author | | Yasuhara, Kazuki Browse this author | | Seki, Hideshi Browse this author →KAKEN DB | | Maruyama, Hideo Browse this author →KAKEN DB |
| Issue Date: | 15-Sep-2002 |
| Publisher: | Elsevier |
| Journal Title: | Journal of Colloid and Interface Science |
| Volume: | 253 |
| Issue: | 2 |
| Start Page: | 402 |
| End Page: | 408 |
| Publisher DOI: | 10.1006/jcis.2002.8526 |
| PMID: | 16290871 |
| Abstract: | A fundamental study about the selective foam separation of protein mixture was carried out. A solution containing two proteins, Ovalbumin (OA) and Lysozyme (LZ), and an anionic surfactant, Sodium Dodecyle Sulfate (SDS), was adjusted to pH 6.0 which refered to an intermediate state between the isoelectric points of the proteins. The solution was processed by continuous foam separation. The results showed that a proper addition of SDS greatly improved the selective recovery of LZ to OA. The experimental data were well explained by a simple model that most of cationic protein molecules (LZ) are associated with SDS and the adsorption of all the species including LZ-SDS complexes are subjected to Langmuir adsorption isotherm. The results also showed that one of the Langmuir parameters, which means a kind of lyophillic property of adsorbed material, of LZ-SDS complexes was extremely large as compared with that of primary protein. |
| Rights: | Copyright © 2002 Elsevier |
| Relation: | http://www.sciencedirect.com/science/journal/00219797 |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/13481 |
| Appears in Collections: | 水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 丸山 英男
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