HUSCAP logo Hokkaido Univ. logo

Hokkaido University Collection of Scholarly and Academic Papers >
水産科学院・水産科学研究院  >
雑誌発表論文等  >

Cloning and characterization of a cDNA encoding cholesterol side-chain cleavage cytochrome P450 (CYP11A1) : Tissue-distribution and changes in the transcript abundance in ovarian tissue of Japanese eel, Anguilla japonica, during artificially induced sexual development

フルテキスト
JSBMB2005-8967.pdf465.67 kBPDF見る/開く
この文献へのリンクには次のURLを使用してください:http://hdl.handle.net/2115/14404

タイトル: Cloning and characterization of a cDNA encoding cholesterol side-chain cleavage cytochrome P450 (CYP11A1) : Tissue-distribution and changes in the transcript abundance in ovarian tissue of Japanese eel, Anguilla japonica, during artificially induced sexual development
著者: Kazeto, Yukinori 著作を一覧する
Ijiri, Shigeho 著作を一覧する
Adachi, Shinji 著作を一覧する
Yamauchi, Kohei 著作を一覧する
キーワード: P450scc
CYP11A1
Steroidogenic enzyme
Ovary
Head kidney
Japanese eel
発行日: 2006年 5月
出版者: Elsevier
誌名: The Journal of Steroid Biochemistry and Molecular Biology
巻: 99
号: 2-3
開始ページ: 121
終了ページ: 128
出版社 DOI: 10.1016/j.jsbmb.2005.12.004
抄録: Cholesterol side chain cleavage cytochrome P450 (CYP11A1: P450scc) is a crucial steroidogenic enzyme that catalyzes an initial step in the production of all classes of steroids. A cDNA encoding Japanese eel P450scc was cloned and characterized. The cDNA putatively encoded 521 amino acid residues with high homology to those of other vertebrate forms. The recombinant P450scc produced in COS-7 cells efficiently catalyzed the conversion of 25-hydroxycholesterol into pregnenolone. By northern blot, a single P450scc transcript of approximately 3.3 kb was detected in both ovary and head kidney. Transcript levels of this enzyme significantly increased throughout ovarian development artificially induced by salmon pituitary homogenate, which suggests that gonadotropic stimuli can induce ovarian expression of the P450scc gene in teleosts, as has been reported in mammals. Furthermore, RT-PCR analysis revealed that gene expression of three steroidogenic enzymes, P450scc, P450c17 and 3β-hydroxysteroid dehydrogenase (3β-HSD) show distinctly different tissue specific patterns of expression in the Japanese eel. The P450scc gene was expressed in ovary and head kidney while the sole source of the P450c17 transcript was ovary. In contrast, 3β-HSD transcript was detected in all tissues examined, brain, liver, spleen and trunk kidney, etc. These suggest that some steroidogenic enzymes are also expressed in non-endocrine tissues and could potentially regulate the local and/or circulating steroid levels in teleosts, as they do in mammals.
関連URI: http://www.sciencedirect.com/science/journal/09600760
資料タイプ: article (author version)
URI: http://hdl.handle.net/2115/14404
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 風藤 行紀

 

本サイトに関するご意見・お問い合わせは repo at lib.hokudai.ac.jp へお願いします。 - 北海道大学