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Mutational analysis of the Lem3p-Dnf1p putative phospholipid-translocating P-type ATPase reveals novel regulatory roles for Lem3p and a carboxyl-terminal region of Dnf1p independent of the phospholipid-translocating activity of Dnf1p in yeast

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タイトル: Mutational analysis of the Lem3p-Dnf1p putative phospholipid-translocating P-type ATPase reveals novel regulatory roles for Lem3p and a carboxyl-terminal region of Dnf1p independent of the phospholipid-translocating activity of Dnf1p in yeast
著者: Noji, T. 著作を一覧する
Yamamoto, T. 著作を一覧する
Saito, K. 著作を一覧する
Fujimura-Kamada, K. 著作を一覧する
発行日: 2006年 5月26日
出版者: ACADEMIC PRESS INC ELSEVIER SCIENCE
誌名: Biochemical and Biophysical Research Communications
巻: 344
号: 1
開始ページ: 323
終了ページ: 331
出版社 DOI: 10.1016/j.bbrc.2006.03.095
抄録: Lem3p-Dnf1p is a putative aminophospholipid translocase (APLT) complex that is localized to the plasma membrane; Lem3p is required for Dnf1p localization to the plasma membrane. We have identified lem3 mutations, which did not affect formation or localization of the Lem3p-Dnf1p complex, but caused a synthetic growth defect with the null mutation of CDC50, a structurally and functionally redundant homologue of LEM3. Interestingly, these lem3 mutants exhibited nearly normal levels of NBD-labeled phospholipid internalization across the plasma membrane, suggesting that Lem3p may have other functions in addition to regulation of the putative APLT activity of Dnf1p at the plasma membrane. Similarly, deletion of the COOH-terminal cytoplasmic region of Dnf1p affected neither the localization nor the APLT activity of Dnf1p at the plasma membrane, but caused a growth defect in the cdc50 Delta background. Our results suggest that the Lem3p-Dnf1p complex may play a role distinct from its plasma membrane APLT activity when it Substitutes for the Cdc50p-Drs2p complex, its redundant partner in the endosomal/trans-Golgi network compartments.
資料タイプ: article (author version)
URI: http://hdl.handle.net/2115/14477
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 山本 隆晴

 

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