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Microsatellite-centromere mapping in the Japanese eel (Anguilla japonica) by half-tetrad analysis using induced triploid families

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Title: Microsatellite-centromere mapping in the Japanese eel (Anguilla japonica) by half-tetrad analysis using induced triploid families
Authors: Nomura, Kazuharu1 Browse this author
Morishima, Kagayaki2 Browse this author
Tanaka, Hideki Browse this author
Unuma, Tatsuya Browse this author
Okuzawa, Koichi Browse this author
Ohta, hiromi Browse this author
Arai, Katsutoshi Browse this author →KAKEN DB
Authors(alt): 野村, 和晴1
森島, 輝2
Keywords: Anguilla japonica
gene-centromere mapping
half-tetrad analysis
interference
microsatellite
triploid
Issue Date: 30-Jun-2006
Publisher: Elsevier
Journal Title: Aquaculture
Volume: 257
Issue: 1-4
Start Page: 53
End Page: 67
Publisher DOI: 10.1016/j.aquaculture.2006.03.011
Abstract: Genetic improvement of the Japanese eel (Anguilla japonica) can be achieved by artificially controlling its life cycle using recent advances in reproductive biology. In this study, we developed 43 microsatellite loci to confirm Mendelian inheritance at 10 of them as well at 16 previously reported in two full-sib families produced by artificial insemination. In order to establish a base for aquaculture genetics of this species in the near future, these microsatellite loci were mapped in relation to the centromere by half-tetrad analysis using four artificially induced triploid families. The second division segregation frequency (y) of the microsatellite loci ranged from 0.008 to 0.968 (mean ± SD = 0.645 ± 0.298). These results suggest the presence of strong chiasma interference in the eel. Significant differences were observed for the map distances of microsatellite loci between the two isolation procedures. Microsatellites isolated using the enrichment procedure were mapped to various sites starting from the centromere to the telomere, whereas those from the conventional size-selected library showed a tendency to be distributed in the telomeric region.
Relation: http://www.sciencedirect.com/science/journal/00448486
Type: article (author version)
URI: http://hdl.handle.net/2115/14487
Appears in Collections:水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 野村 和晴

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