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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences >
Peer-reviewed Journal Articles, etc >
Microsatellite-centromere mapping in the Japanese eel (Anguilla japonica) by half-tetrad analysis using induced triploid families
| Title: | Microsatellite-centromere mapping in the Japanese eel (Anguilla japonica) by half-tetrad analysis using induced triploid families |
| Authors: | Nomura, Kazuharu1 Browse this author | | Morishima, Kagayaki2 Browse this author | | Tanaka, Hideki Browse this author | | Unuma, Tatsuya Browse this author | | Okuzawa, Koichi Browse this author | | Ohta, hiromi Browse this author | | Arai, Katsutoshi Browse this author →KAKEN DB |
| Authors(alt): | 野村, 和晴1 | | 森島, 輝2 |
| Keywords: | Anguilla japonica | | gene-centromere mapping | | half-tetrad analysis | | interference | | microsatellite | | triploid |
| Issue Date: | 30-Jun-2006 |
| Publisher: | Elsevier |
| Journal Title: | Aquaculture |
| Volume: | 257 |
| Issue: | 1-4 |
| Start Page: | 53 |
| End Page: | 67 |
| Publisher DOI: | 10.1016/j.aquaculture.2006.03.011 |
| Abstract: | Genetic improvement of the Japanese eel (Anguilla japonica) can be achieved by artificially controlling its life cycle using recent advances in reproductive biology. In this study, we developed 43 microsatellite loci to confirm Mendelian inheritance at 10 of them as well at 16 previously reported in two full-sib families produced by artificial insemination. In order to establish a base for aquaculture genetics of this species in the near future, these microsatellite loci were mapped in relation to the centromere by half-tetrad analysis using four artificially induced triploid families. The second division segregation frequency (y) of the microsatellite loci ranged from 0.008 to 0.968 (mean ± SD = 0.645 ± 0.298). These results suggest the presence of strong chiasma interference in the eel. Significant differences were observed for the map distances of microsatellite loci between the two isolation procedures. Microsatellites isolated using the enrichment procedure were mapped to various sites starting from the centromere to the telomere, whereas those from the conventional size-selected library showed a tendency to be distributed in the telomeric region. |
| Relation: | http://www.sciencedirect.com/science/journal/00448486 |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/14487 |
| Appears in Collections: | 水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 野村 和晴
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