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Ammonia Channel Couples Glutaminase with Transamidase Reactions in GatCAB

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Title: Ammonia Channel Couples Glutaminase with Transamidase Reactions in GatCAB
Authors: Nakamura, Akiyoshi1 Browse this author
Yao, Min Browse this author →KAKEN DB
Chimnaronk, Sarin Browse this author
Sakai, Naoki Browse this author
Tanaka, Isao5 Browse this author →KAKEN DB
Authors(alt): 中村, 彰良1
田中, 勲5
Issue Date: 30-Jun-2006
Publisher: American Association for the Advancement of Science
Journal Title: Science
Volume: 312
Issue: 5782
Start Page: 1954
End Page: 1958
Publisher DOI: 10.1126/science.1127156
PMID: 16809541
Abstract: The formation of glutaminyl. transfer RNA (Gln-tRNA(Gln)) differs among the three domains of life. Most bacteria employ an indirect pathway to produce Gln-tRNA(Gln) by a heterotrimeric glutamine amidotransferase CAB (GatCAB) that acts on the misacylated Glu-tRNA(Gln). Here, we describe a series of crystal structures of intact GatCAB from Staphylococcus aureus in the apo form and in the complexes with glutamine, asparagine, Mn2+, and adenosine triphosphate analog. Two identified catalytic centers for the glutaminase and transamidase reactions are markedly distant but connected by a hydrophilic ammonia channel 30 angstrom in length. Further, we show that the first U-A base pair in the acceptor stem and the D loop of tRNA(Gln) serve as identity elements essential for discrimination by GatCAB and propose a complete model for the overall concerted reactions to synthesize Gin-tRNA(Gln).
Type: article
Appears in Collections:生命科学院・先端生命科学研究院 (Graduate School of Life Science / Faculty of Advanced Life Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 中村 彰良

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