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Hokkaido University Collection of Scholarly and Academic Papers >
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Ammonia Channel Couples Glutaminase with Transamidase Reactions in GatCAB
| Title: | Ammonia Channel Couples Glutaminase with Transamidase Reactions in GatCAB |
| Authors: | Nakamura, Akiyoshi1 Browse this author | | Yao, Min Browse this author →KAKEN DB | | Chimnaronk, Sarin Browse this author | | Sakai, Naoki Browse this author | | Tanaka, Isao5 Browse this author →KAKEN DB |
| Authors(alt): | 中村, 彰良1 | | 田中, 勲5 |
| Issue Date: | 30-Jun-2006 |
| Publisher: | American Association for the Advancement of Science |
| Journal Title: | Science |
| Volume: | 312 |
| Issue: | 5782 |
| Start Page: | 1954 |
| End Page: | 1958 |
| Publisher DOI: | 10.1126/science.1127156 |
| PMID: | 16809541 |
| Abstract: | The formation of glutaminyl. transfer RNA (Gln-tRNA(Gln)) differs among the three domains of life. Most bacteria employ an indirect pathway to produce Gln-tRNA(Gln) by a heterotrimeric glutamine amidotransferase CAB (GatCAB) that acts on the misacylated Glu-tRNA(Gln). Here, we describe a series of crystal structures of intact GatCAB from Staphylococcus aureus in the apo form and in the complexes with glutamine, asparagine, Mn2+, and adenosine triphosphate analog. Two identified catalytic centers for the glutaminase and transamidase reactions are markedly distant but connected by a hydrophilic ammonia channel 30 angstrom in length. Further, we show that the first U-A base pair in the acceptor stem and the D loop of tRNA(Gln) serve as identity elements essential for discrimination by GatCAB and propose a complete model for the overall concerted reactions to synthesize Gin-tRNA(Gln). |
| Relation: | http://www.sciencemag.org/cgi/content/full/312/5782/1954 |
| Type: | article |
| URI: | http://hdl.handle.net/2115/14490 |
| Appears in Collections: | 生命科学院・先端生命科学研究院 (Graduate School of Life Science / Faculty of Advanced Life Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 中村 彰良
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