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From phasing to structure refinement in-house : Cr/Cu dual-wavelength system and a loopless free crystal-mounting method
| Title: | From phasing to structure refinement in-house : Cr/Cu dual-wavelength system and a loopless free crystal-mounting method |
| Authors: | Watanabe, Nobuhisa Browse this author |
| Keywords: | Cr radiation | | SAD method | | free crystal mounting method |
| Issue Date: | Aug-2006 |
| Publisher: | International Union of Crystallography |
| Journal Title: | Acta Crystallographica Section D: Biological Crystallography |
| Volume: | 62 |
| Issue: | 8 |
| Start Page: | 891 |
| End Page: | 896 |
| Publisher DOI: | 10.1107/S0907444906010432 |
| PMID: | 16855305 |
| Abstract: | The practical applicability of in-house structure determination using Cr Kα X-rays (2.29 Å) and a loopless free crystal mounting method was examined using five novel proteins. Proteins from 9.6 kDa to 84 kDa have been solved using this method without any derivatization. In all cases, more than 90% of all the structures were constructed automatically with side chains by use of the Cr SAD method. The free crystal mounting technique increases the accuracy of the anomalous differences between the Bijvoet mates, and makes the in-house singlewavelength SAD method with a Cr Kα X-ray source a very useful tool for high-throughput structure determination. In addition, a Cr/Cu dual wavelength system makes it possible to perform structure analysis from phasing to refinement of the structure in-house. |
| Rights: | Copyright © International Union of Crystallography |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/14526 |
| Appears in Collections: | 生命科学院・先端生命科学研究院 (Graduate School of Life Science / Faculty of Advanced Life Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 渡邉 信久
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