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Cloning and functional characterization of a novel up-regulator, cartregulin, of carnitine transporter, OCTN2

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Title: Cloning and functional characterization of a novel up-regulator, cartregulin, of carnitine transporter, OCTN2
Authors: Nagai, Kazuhiko Browse this author
Takikawa, Osamu Browse this author
Kawakami, Naoko Browse this author
Fukao, Mitsuhiro Browse this author
Soma, Takamitsu Browse this author
Oda, Atsushi Browse this author
Nishiya, Tadashi Browse this author
Hayashi, Misato Browse this author
Lu, Lingyun Browse this author
Nakano, Manabu Browse this author
Kajita, Emi Browse this author
Fujita, Hiroyoshi Browse this author
Miwa, Soichi Browse this author →KAKEN DB
Keywords: cDNA cloning
Acetylcarnitine
Carnitine
OCTN2
mRNA stabilization
Transporter
Up-regulation
Alzheimer’s disease
Issue Date: 1-Aug-2006
Publisher: Elsevier Inc.
Journal Title: Archives of Biochemistry and Biophysics
Volume: 452
Issue: 1
Start Page: 29
End Page: 37
Publisher DOI: 10.1016/j.abb.2006.06.006
PMID: 16839516
Abstract: Acetylcarnitine exerts therapeutic effects on some neurological disorders including Alzheimer’s disease. OCTN2 is known as a transporter for acetylcarnitine, but its expression in the brain is very low. To examine a brain-specific transporter for acetylcarnitine, we screened a rat brain cDNA library by hybridization using a DNA probe conserved among an OCTN family. A cDNA homologous to OCTN2 cDNA was isolated. The cDNA encoded a novel 146-amino acid protein with one putative transmembrane domain. The mRNA was expressed not only in rat brain but also in some other tissues. The novel protein was localized in endoplasmic reticulum when expressed in COS-7 cells but exhibited no transport activity for acetylcarnitine. However, when co-expressed with OCTN2, it enhanced the OCTN2-mediated transport by about twofold. The enhancement was accompanied by an increase in the levels of mRNA and protein. When OCTN2 was expressed in Xenopus oocytes by injection of its cRNA, its transport activity was enhanced by co-expression of the novel protein. These data suggest that the novel protein increases OCTN2 by stabilizing the mRNA in endoplasmic reticulum. The protein may be an up-regulator of OCTN2 and is tentatively designated cartregulin.
Relation: http://www.sciencedirect.com/science/journal/00039861
Type: article (author version)
URI: http://hdl.handle.net/2115/14620
Appears in Collections:医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 三輪 聡一

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