|
Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Environmental Science / Faculty of Environmental Earth Science >
Peer-reviewed Journal Articles, etc >
A phosphopantetheinyl transferase gene essential for biosynthesis of n-3 polyunsaturated fatty acids from Moritella marina strain MP-1
| Title: | A phosphopantetheinyl transferase gene essential for biosynthesis of n-3 polyunsaturated fatty acids from Moritella marina strain MP-1 |
| Authors: | Orikasa, Yoshitake Browse this author | | Nishida, Takanori Browse this author | | Hase, Akira Browse this author | | Watanabe, Kazuo Browse this author | | Morita, Naoki Browse this author | | Okuyama, Hidetoshi Browse this author →KAKEN DB |
| Keywords: | Docosahexaenoic acid | | Eicosapentaenoic acid | | n−3 Polyunsaturated fatty acid | | Phosphopantetheinyl transferase | | pfaE gene | | Moritella marina strain MP-1 |
| Issue Date: | 7-Aug-2006 |
| Publisher: | Elsevier B.V. |
| Journal Title: | FEBS Letters |
| Volume: | 580 |
| Issue: | 18 |
| Start Page: | 4423 |
| End Page: | 4429 |
| Publisher DOI: | 10.1016/j.febslet.2006.07.008 |
| PMID: | 16859689 |
| Abstract: | A phosphopantetheinyl transferase (PPTase) gene (pfaE), cloned from the docosahexaenoic acid (DHA)-producing bacterium Moritella marina strain MP-1, has an open reading frame of 861 bp encoding a 287-amino acid protein. When the pfaE gene was expressed with pfaA–D, which are four out of five essential genes for biosynthesis of eicosapentaenoic acid (EPA) derived from Shewanella pneumatophori SCRC-2738 in Escherichia coli, the recombinant produced 12% EPA of total fatty acids. This suggests that pfaE encodes a PPTase required for producing n−3 polyunsaturated fatty acids, which is probably involved in the synthesis of DHA in M. marina strain MP-1. |
| Relation: | http://www.sciencedirect.com/science/journal/00145793 |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/14652 |
| Appears in Collections: | 環境科学院・地球環境科学研究院 (Graduate School of Environmental Science / Faculty of Environmental Earth Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
|
Submitter: 奥山 英登志
|
