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A phosphopantetheinyl transferase gene essential for biosynthesis of n-3 polyunsaturated fatty acids from Moritella marina strain MP-1

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Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/14652

Title: A phosphopantetheinyl transferase gene essential for biosynthesis of n-3 polyunsaturated fatty acids from Moritella marina strain MP-1
Authors: Orikasa, Yoshitake Browse this author
Nishida, Takanori Browse this author
Hase, Akira Browse this author
Watanabe, Kazuo Browse this author
Morita, Naoki Browse this author
Okuyama, Hidetoshi Browse this author →KAKEN DB
Keywords: Docosahexaenoic acid
Eicosapentaenoic acid
n−3 Polyunsaturated fatty acid
Phosphopantetheinyl transferase
pfaE gene
Moritella marina strain MP-1
Issue Date: 7-Aug-2006
Publisher: Elsevier B.V.
Journal Title: FEBS Letters
Volume: 580
Issue: 18
Start Page: 4423
End Page: 4429
Publisher DOI: 10.1016/j.febslet.2006.07.008
PMID: 16859689
Abstract: A phosphopantetheinyl transferase (PPTase) gene (pfaE), cloned from the docosahexaenoic acid (DHA)-producing bacterium Moritella marina strain MP-1, has an open reading frame of 861 bp encoding a 287-amino acid protein. When the pfaE gene was expressed with pfaA–D, which are four out of five essential genes for biosynthesis of eicosapentaenoic acid (EPA) derived from Shewanella pneumatophori SCRC-2738 in Escherichia coli, the recombinant produced 12% EPA of total fatty acids. This suggests that pfaE encodes a PPTase required for producing n−3 polyunsaturated fatty acids, which is probably involved in the synthesis of DHA in M. marina strain MP-1.
Relation: http://www.sciencedirect.com/science/journal/00145793
Type: article (author version)
URI: http://hdl.handle.net/2115/14652
Appears in Collections:環境科学院・地球環境科学研究院 (Graduate School of Environmental Science / Faculty of Environmental Earth Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 奥山 英登志

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