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Production of phosphatidylcholine containing conjugated linoleic acid mediated by phospholipase A2

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Title: Production of phosphatidylcholine containing conjugated linoleic acid mediated by phospholipase A2
Authors: Yamamoto, Yukihiro Browse this author
Hosokawa, Masashi Browse this author →KAKEN DB
Miyashita, Kazuo Browse this author →KAKEN DB
Keywords: Phosphatidylcholine
Conjugated linoleic acid
Phosphalipase A2
Water mimic
Issue Date: 4-Aug-2006
Publisher: Elsevier B.V.
Journal Title: Journal of Molecular Catalysis B Enzymatic
Volume: 41
Issue: 3-4
Start Page: 92
End Page: 96
Publisher DOI: 10.1016/j.molcatb.2006.04.014
Abstract: Esterification of lysophosphatidylcholine (LPC) with conjugated linoleic acid (CLA) was carried out using porcine pancreatic phospholipase A2 (PLA2). PLA2 only slightly synthesized phosphatidylcholine containing CLA (CLA-PC) at 2.6% by the addition of water. Addition of formamide in place of water markedly increased the yield of CLA-PC. In addition, synthesis of CLA-PC by PLA2 was affected by the amount of substrate CLA and PLA2 in the reaction system. Under optimal reaction conditions using 11 mg LPC, 18 mg CLA, 550 mg glycerol, 50 μL formamide, 3.3 × 104 U PLA2, and 0.3 μmol CaCl2 at 37 °C for 6 h, the reaction yield of CLA-PC reached 65 mol%. Furthermore, addition of protein such as albumin and casein suppressed the decrease of CLA-PC yield after 6 h. PLA2 exhibited the highest activity for the 10t,12c-CLA isomer among four CLA isomers (9c,11t-CLA, 9c,11c-CLA, 9t,11t-CLA and 10t,12c-CLA), whereas that for 9c,11c-CLA was the lowest. These results showed that the present esterification system for LPC and CLA by PLA2 is effective for producing CLA-PC.
Type: article (author version)
Appears in Collections:水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 細川 雅史

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