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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine >
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In vitro demonstration of neural transmission of avian influenza A virus
| Title: | In vitro demonstration of neural transmission of avian influenza A virus |
| Authors: | Matsuda, Kazuya Browse this author | | Shibata, Takuma Browse this author | | Sakoda, Yoshihiro Browse this author →KAKEN DB | | Kida, Hiroshi Browse this author →KAKEN DB | | Kimura, Takashi Browse this author →KAKEN DB | | Ochiai, Kenji Browse this author →KAKEN DB | | Umemura, Takashi Browse this author →KAKEN DB |
| Issue Date: | Apr-2005 |
| Publisher: | Society for General Microbiology |
| Journal Title: | Journal of General Virology |
| Volume: | 86 |
| Issue: | 4 |
| Start Page: | 1131 |
| End Page: | 1139 |
| Publisher DOI: | 10.1099/vir.0.80704-0 |
| PMID: | 15784907 |
| Abstract: | Neural involvement following infections of influenza viruses can be serious. The neural transport of influenza viruses from the periphery to the central nervous system has been indicated by using mouse models. However, no direct evidence for neuronal infection has been obtained in vitro and the mechanisms of neural transmission of influenza viruses have not been reported. In this study, the transneural transmission of a neurotropic influenza A virus was examined using compartmentalized cultures of neurons from mouse dorsal root ganglia, and the results were compared with those obtained using the pseudorabies virus, a virus with well-established neurotransmission. Both viruses reached the cell bodies of the neurons via the axons. This is the first report on axonal transport of influenza A virus in vitro. In addition, the role of the cytoskeleton (microtubules, microfilaments and intermediate filaments) in the neural transmission of influenza virus was investigated by conducting cytoskeletal perturbation experiments. The results indicated that the transport of avian influenza A virus in the neurons was independent of microtubule integrity but was dependent on the integrity of intermediate filaments, whereas pseudorabies virus needed both for neural spread. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/14697 |
| Appears in Collections: | 獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 梅村 孝司
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