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PDGF isoforms induce and maintain anagen phase of murine hair follicles
|タイトル: ||PDGF isoforms induce and maintain anagen phase of murine hair follicles|
|著者: ||Tomita, Y. 著作を一覧する|
Akiyama, M. 著作を一覧する
Shimizu, H. 著作を一覧する
|キーワード: ||Hair cycle|
|発行日: ||2006年 8月|
|出版者: ||Elsevier Ireland|
|誌名: ||Journal of Dermatological Science|
|出版社 DOI: ||10.1016/j.jdermsci.2006.03.012|
|抄録: ||Background : It is known that platelet-derived growth factor (PDGF) receptors are expressed in hair follicle (HF) epithelium.
Objectives : The aim of the present study was to clarify the effects of PDGF-AA and -BB on the cyclic growth of HFs.
Methods : PDGF-AA or -BB was injected into the dorsal skin of C3H mice during the second telogen phase once daily for five consecutive days, or PDGF-AA or -BB dissolved in hyaluronic acid was injected only once. In order to confirm the effects of different PDGF isoforms, anti-PDGF-AA antibody or anti-PDGF-BB antibody was injected just after each injection of PDGF-AA or -BB. In addition, anti-PDGF antibodies were injected into the skin of C3H mice during the second anagen phase once daily for 5 days. We studied expression of signaling molecules in the skin where anagen phase had been induced by PDGF injection by real-time RT-PCR.
Results : Both PDGF-AA and -BB injection experiments immediately induced the anagen phase of the hair growth cycle at the injection sites. The induction of anagen was interfered by anti-PDGF antibody treatment. Real-time RT-PCR using extracted RNA from the PDGF injected sites of skin samples showed upregulated expression of HF differentiation-related key signaling molecules, Sonic hedgehog (Shh), Lef-1 and Wnt5a.
Conclusions : These results indicate that both PDGF-AA and -BB are involved in the induction and maintenance of the anagen phase in the mouse hair cycle. Local application of PDGF-AA and -BB might therefore prove to be an effective treatment option for alopecia associated with early catagen induction and elongated telogen phase.|
|Relation (URI): ||http://www.sciencedirect.com/science/journal/09231811|
|資料タイプ: ||article (author version)|
|出現コレクション:||雑誌発表論文等 (Peer-reviewed Journal Articles, etc)|
提供者: 秋山 真志