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PDGF isoforms induce and maintain anagen phase of murine hair follicles
| Title: | PDGF isoforms induce and maintain anagen phase of murine hair follicles |
| Authors: | Tomita, Y. Browse this author | | Akiyama, M. Browse this author | | Shimizu, H. Browse this author →KAKEN DB |
| Keywords: | Hair cycle | | Hair growth | | PDGF-AA | | PDGF-BB |
| Issue Date: | Aug-2006 |
| Publisher: | Elsevier Ireland |
| Journal Title: | Journal of Dermatological Science |
| Volume: | 43 |
| Issue: | 2 |
| Start Page: | 105 |
| End Page: | 115 |
| Publisher DOI: | 10.1016/j.jdermsci.2006.03.012 |
| PMID: | 16725313 |
| Abstract: | Background : It is known that platelet-derived growth factor (PDGF) receptors are expressed in hair follicle (HF) epithelium.
Objectives : The aim of the present study was to clarify the effects of PDGF-AA and -BB on the cyclic growth of HFs.
Methods : PDGF-AA or -BB was injected into the dorsal skin of C3H mice during the second telogen phase once daily for five consecutive days, or PDGF-AA or -BB dissolved in hyaluronic acid was injected only once. In order to confirm the effects of different PDGF isoforms, anti-PDGF-AA antibody or anti-PDGF-BB antibody was injected just after each injection of PDGF-AA or -BB. In addition, anti-PDGF antibodies were injected into the skin of C3H mice during the second anagen phase once daily for 5 days. We studied expression of signaling molecules in the skin where anagen phase had been induced by PDGF injection by real-time RT-PCR.
Results : Both PDGF-AA and -BB injection experiments immediately induced the anagen phase of the hair growth cycle at the injection sites. The induction of anagen was interfered by anti-PDGF antibody treatment. Real-time RT-PCR using extracted RNA from the PDGF injected sites of skin samples showed upregulated expression of HF differentiation-related key signaling molecules, Sonic hedgehog (Shh), Lef-1 and Wnt5a.
Conclusions : These results indicate that both PDGF-AA and -BB are involved in the induction and maintenance of the anagen phase in the mouse hair cycle. Local application of PDGF-AA and -BB might therefore prove to be an effective treatment option for alopecia associated with early catagen induction and elongated telogen phase. |
| Relation: | http://www.sciencedirect.com/science/journal/09231811 |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/14708 |
| Appears in Collections: | 北海道大学病院 (Hokkaido University Hospital) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 秋山 真志
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