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Isolation and characterization of polymorphic microsatellite DNA markers in two shrew species, Sorex unguiculatus and S. caecutiens

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Title: Isolation and characterization of polymorphic microsatellite DNA markers in two shrew species, Sorex unguiculatus and S. caecutiens
Authors: Naitoh, Y. Browse this author
Ishibashi, Y. Browse this author
Abe, S. Browse this author →KAKEN DB
Ohdachi, S. D.4 Browse this author →KAKEN DB
Authors(alt): 大舘, 智志4
Keywords: cross-species amplification
shrew
simple sequence repeat
Sorex
Soricidae
microsatellites
Issue Date: 2002
Publisher: Blackwell Publishing
Journal Title: Molecular Ecology Notes
Volume: 2
Issue: 4
Start Page: 434
End Page: 436
Abstract: We isolated six microsatellite markers from the partial genomic libraries of two Sorex shrews, S. unguiculatus and S. caecutiens, and examined their allelic variation. All loci showed high allelic variation ranging from 15 to 19 alleles and all but one locus conformed to Hardy-Weinberg expectations in the species where the loci were isolated. Cross-species amplifications showed that all primers derived from S. unguiculatus were useful for S. caecutiens, while among primer sets derived from S. caecutiens, only one was useful for S. unguiculatus. Accordingly, at least five microsatellite markers were useful in S. caecutiens and three in S. unguiculatus.
Rights: Copyright 2002 Blackwell Publishing
Type: article (author version)
URI: http://hdl.handle.net/2115/1471
Appears in Collections:低温科学研究所 (Institute of Low Temperature Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 大舘 智志

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