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Captopril suppresses inflammation in endotoxin-induced uveitis in rats

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タイトル: Captopril suppresses inflammation in endotoxin-induced uveitis in rats
著者: Ilieva, Iliyana 著作を一覧する
Ohgami, Kazuhiro 著作を一覧する
Jin, Xue-Hai 著作を一覧する
Suzuki, Yukari 著作を一覧する
Shiratori, Kenji 著作を一覧する
Yoshida, Kazuhiko 著作を一覧する
Kase, Satoru 著作を一覧する
Ohno, Shigeaki 著作を一覧する
キーワード: captopril
rennin-angiotensin system
anti-inflammatory agent
発行日: 2006年 9月
出版者: Elsevier B.V.
誌名: Experimental Eye Research
巻: 83
号: 3
開始ページ: 651
終了ページ: 657
出版社 DOI: 10.1016/j.exer.2006.03.005
抄録: Captopril is an inhibitor of angiotensin-converting enzyme (ACE) that is largely used in the treatment of cardiovascular diseases. Several previous studies have demonstrated that captopril exhibits a wide variety of biological activities, including an anti-inflammatory action, on which we focused our attention. The aim of the present study was to investigate the efficacy of captopril on endotoxin induced uveitis (EIU) in rats. We investigated its effect upon cellular infiltration and protein leakage, as well as on the concentration of tumor necrosis factor-α (TNF-α), nitric oxide (NO), prostaglandin E2 (PGE2), monocyte chemoattractant protein-1 (MCP-1) in the anterior chamber. In addition, we checked its effect on activation of nuclear factor kappa B (NF-κB) in iris and ciliary body (ICB) cells in vivo. EIU was induced in male Lewis rats by a footpad injection of lipopolysaccharide (LPS). One hour after the LPS inoculation, either 1 mg/kg, 10 mg/kg or 100 mg/kg captopril were injected intravenously. 24 h later, the aqueous humor was collected from both eyes, and the number of infiltrating cells and protein concentration in the aqueous humor were determined. Levels of TNF-α, PGE2, NO and MCP-1 were determined by enzyme-linked immunosorbent assay. On some eyes, after enucleation, immunohistochemical staining with a monoclonal antibody against activated NF-κB was performed. Captopril treatment significantly decreased the inflammatory cells infiltration, the level of protein, concentrations of TNF-α, PGE2, NO and MCP-1 in the aqueous humor. The number of activated NF-κB-positive cells was lower in ICB of the rats treated with captopril 3 h after the LPS injection. The present results indicate that captopril suppresses the inflammation in EIU by inhibiting the NF-κB-dependent pathway and the subsequent production of pro-inflammatory mediators.
Relation (URI):
資料タイプ: article (author version)
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)



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