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Activation process of the mosquitocidal δ-endotoxin Cry39A produced by Bacillus thuringiensis subsp aizawai BUN1-14 and binding property to Anopheles stephensi BBMV

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Title: Activation process of the mosquitocidal δ-endotoxin Cry39A produced by Bacillus thuringiensis subsp aizawai BUN1-14 and binding property to Anopheles stephensi BBMV
Authors: Ito, Takeshi Browse this author
Bando, Hisanori Browse this author →KAKEN DB
Asano, Shin-ichiro Browse this author →KAKEN DB
Keywords: Bacillus thuringiensis
δ-Endotoxin; Mosquito
Anopheles stephensi
Processing; BBMV
Issue Date: Sep-2006
Publisher: Elsevier B.V.
Journal Title: Journal of Invertebrate Pathology
Volume: 93
Issue: 1
Start Page: 29
End Page: 35
Publisher DOI: 10.1016/j.jip.2006.05.007
PMID: 16837008
Abstract: Most δ-endotoxins produced by Bacillus thuringiensis require proteolytic processing in order to become active. The in vitro and in vivo activation processes of Cry39A, a δ-endotoxin that is highly toxic to Anopheles stephensi, were investigated. Cry39A with a molecular mass of 72 kDa was processed in vitro into a 60 kDa fragment by trypsin and gut extract from A. stephensi larvae. N-terminal amino acid sequencing of the 60 kDa fragment revealed that trypsin and the protease(s) in the gut extract cleaved Cry39A between Arg61 and Gly62. In contrast, 40 and 25 kDa polypeptides were generated in vivo by intramolecular cleavage of the 60 kDa fragment in A. stephensi larvae. Further, a co-precipitation assay was used to investigate the binding property of the activated Cry39A to A. stephensi BBMV. Cry39A bound to A. stephensi BBMV specifically and did not compete with the Cry4Aa toxin. This indicated that the binding molecule(s) for Cry39A might differ from those for Cry4A. In addition, Cry39A preferentially bound to the Triton X-100-insoluble membrane fraction.
Relation: http://www.sciencedirect.com/science/journal/00222011
Type: article (author version)
URI: http://hdl.handle.net/2115/14743
Appears in Collections:農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 浅野 眞一郎

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