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Effect of lycopene and beta-carotene on peroxynitrite-mediated cellular modifications.

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Title: Effect of lycopene and beta-carotene on peroxynitrite-mediated cellular modifications.
Authors: Muzandu, Kaampwe Browse this author
Ishizuka, Mayumi Browse this author →KAKEN DB
Sakamoto, Kentaro Q. Browse this author →KAKEN DB
Shaban, Zein Browse this author
El Bohi, Khlood Browse this author
Kazusaka, Akio Browse this author →KAKEN DB
Fujita, Shoichi Browse this author →KAKEN DB
Keywords: DNA damage
Protein tyrosine nitration
Issue Date: 15-Sep-2006
Publisher: Elsevier Inc.
Journal Title: Toxicology and Applied Pharmacology
Volume: 215
Issue: 3
Start Page: 330
End Page: 340
Publisher DOI: 10.1016/j.taap.2006.03.006
PMID: 16647730
Abstract: Peroxynitrite formed by the reaction of superoxide and nitric oxide is a highly reactive species with a role in various pathological processes such as cancer, chronic inflammation, and cardiovascular and neurological diseases. In the present study, the effect of the carotenoids, lycopene and β-carotene, on peroxynitrite-mediated modifications in plasmid DNA as well as cellular DNA and proteins were investigated. In pUC18 plasmid DNA, these carotenoids strongly inhibited DNA strand breaks caused by peroxynitrite generated from 3-morpholinosydnonimine (SIN-1). SIN-1 was also used to determine effects on DNA damage and protein tyrosine nitration in Chinese hamster lung fibroblasts. SIN-1 dose-dependently increased nitration of proteins in cells above basal levels as determined by Western blotting. This nitration was inhibited in the presence of the uric acid as well as lycopene. Physiological concentrations (0.31–10 μM) of lycopene and β-carotene also had protective effects on DNA damage, as measured by the comet assay. Lycopene significantly reduced DNA damage particularly, in the median range of concentrations (2.5 μM). The protective effects of lycopene and β-carotene could be due to their scavenging of reactive oxygen (ROS) and/or nitrogen species (RNS) as they reduce the amount of intracellular ROS/RNS produced following treatment with SIN-1 by as much as 47.5% and 42.4%, respectively. The results obtained in this study suggest that carotenoids may alleviate some of the deleterious effects of peroxynitrite and possibly other reactive nitrogen species as well in vivo.
Type: article (author version)
Appears in Collections:獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 藤田 正一

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