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Effects of long-term ingestion of difructose anhydride III (DFA III) on intestinal bacteria and bile acid metabolism in humans

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Title: Effects of long-term ingestion of difructose anhydride III (DFA III) on intestinal bacteria and bile acid metabolism in humans
Authors: Minamida, Kimiko Browse this author
Asakawa, Chikako Browse this author
Sujaya I., Nengah Browse this author
Kaneko, Maki Browse this author
Abe, Ayumi Browse this author
Sone, Teruo Browse this author →KAKEN DB
Hara, Hiroshi Browse this author
Asano, Kozo Browse this author →KAKEN DB
Tomita, Fusao Browse this author
Keywords: difructose anhydride III (DFA III)
denaturing gradient gel electrophoresis (DGGE)
bile acids
Issue Date: Feb-2006
Publisher: 日本生物工学会
Journal Title: Journal of Bioscience and Bioengineering
Volume: 101
Issue: 2
Start Page: 149
End Page: 156
Publisher DOI: 10.1263/jbb.101.149
PMID: 16569611
Abstract: Changes in the intestinal microbiota of 10 human subjects with long-term ingestion of 3 g/d difructose anhydride III (DFA III; 4 persons, 2 months; 3 persons, 6 months; and 3 persons, 12 months) were examined by denaturing gradient gel electrophoresis (DGGE). According to the answers to questionnaires, the subjects were divided into two groups (constipated and normal). The DGGE profile was different for every individual and each subject had unique profiles of intestinal microbiota. In the DGGE profiles of constipated subjects, the intensities of bands related to Bacteroides spp. increased. Moreover, the DFA III-assimilating bacteria, Ruminococcus sp. were isolated from subjects who ingested DFA III for 12 months. These strains showed 95% similarity of their 16S rDNA sequences with that of Ruminococcus obeum ATCC 29174T (X85101) and produced large amounts of acetic acid. DFA III ingestion for 2 months tended to increase total organic acids in feces, and tended to decrease fecal pH and the secondary bile acid (SBA) ratio in total bile acids. The SBA ratio in total bile acids corresponded to fecal pH. The production of SBA was decreased by low pH in vitro. These results indicated that DFA III ingestion in humans tended to lower intestinal pH, inhibited bile acid 7α-dehydroxylation activities and also tended to decrease the SBA ratios in total bile acids. Moreover, as another cause for the decrease in the SBA ratio in total bile acids, it was suggested that the number of bile acid 7α-dehydroxylating bacteria were decreased by DFA III ingestion
Type: article (author version)
Appears in Collections:農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 冨田 房男

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