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Biofilm formation by a Bacillus subtilis strain that produces γ-polyglutamate

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Title: Biofilm formation by a Bacillus subtilis strain that produces γ-polyglutamate
Authors: Morikawa, Masaaki Browse this author →KAKEN DB
Kagihiro, Shinji Browse this author
Haruki, Mitsuru Browse this author
Takano, Kazufumi Browse this author
Branda, Steve Browse this author
Kolter, Roberto Browse this author
Kanaya, Shigenori Browse this author
Issue Date: 2006
Publisher: Society for General Microbiology
Journal Title: Microbiology
Volume: 152
Issue: 9
Start Page: 2801
End Page: 2807
Publisher DOI: 10.1099/mic.0.29060-0
Abstract: The extracellular matrix produced by Bacillus subtilis B-1, an environmental strain that forms robust floating biofilms, was purified, and determined to be composed predominantly of -polyglutamate (-PGA), with a molecular mass of over 1000 kDa. Both biofilm formation and -PGA production by B. subtilis B-1 increased with increasing Mn2+ or glycerol concentration. -PGA was produced in a growth-associated manner in standing culture, and floating biofilms were formed. However, -PGA was produced in a non-growth-associated manner in shaking culture conditions. When B. subtilis B-1 was grown in a microaerated culture system, floating biofilm formation and -PGA production were significantly retarded, suggesting that oxygen depletion is involved in the initial steps of floating biofilm formation in standing culture. Proteomic analysis of membrane proteins demonstrated that flagellin, oligopeptide permease and Vpr protease precursor were the major proteins produced by cells in a floating biofilm and a colony.
Relation: http://mic.sgmjournals.org/
Type: article
URI: http://hdl.handle.net/2115/14776
Appears in Collections:環境科学院・地球環境科学研究院 (Graduate School of Environmental Science / Faculty of Environmental Earth Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 森川 正章

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