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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Environmental Science / Faculty of Environmental Earth Science >
Peer-reviewed Journal Articles, etc >
Biofilm formation by a Bacillus subtilis strain that produces γ-polyglutamate
| Title: | Biofilm formation by a Bacillus subtilis strain that produces γ-polyglutamate |
| Authors: | Morikawa, Masaaki Browse this author →KAKEN DB | | Kagihiro, Shinji Browse this author | | Haruki, Mitsuru Browse this author | | Takano, Kazufumi Browse this author | | Branda, Steve Browse this author | | Kolter, Roberto Browse this author | | Kanaya, Shigenori Browse this author |
| Issue Date: | 2006 |
| Publisher: | Society for General Microbiology |
| Journal Title: | Microbiology |
| Volume: | 152 |
| Issue: | 9 |
| Start Page: | 2801 |
| End Page: | 2807 |
| Publisher DOI: | 10.1099/mic.0.29060-0 |
| Abstract: | The extracellular matrix produced by Bacillus subtilis B-1, an environmental strain that forms robust floating biofilms, was purified, and determined to be composed predominantly of -polyglutamate (-PGA), with a molecular mass of over 1000 kDa. Both biofilm formation and -PGA production by B. subtilis B-1 increased with increasing Mn2+ or glycerol concentration. -PGA was produced in a growth-associated manner in standing culture, and floating biofilms were formed. However, -PGA was produced in a non-growth-associated manner in shaking culture conditions. When B. subtilis B-1 was grown in a microaerated culture system, floating biofilm formation and -PGA production were significantly retarded, suggesting that oxygen depletion is involved in the initial steps of floating biofilm formation in standing culture. Proteomic analysis of membrane proteins demonstrated that flagellin, oligopeptide permease and Vpr protease precursor were the major proteins produced by cells in a floating biofilm and a colony. |
| Relation: | http://mic.sgmjournals.org/ |
| Type: | article |
| URI: | http://hdl.handle.net/2115/14776 |
| Appears in Collections: | 環境科学院・地球環境科学研究院 (Graduate School of Environmental Science / Faculty of Environmental Earth Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 森川 正章
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