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Effects of difructose anhydride III (DFA III) administration on rat intestinal microbiota.

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Title: Effects of difructose anhydride III (DFA III) administration on rat intestinal microbiota.
Authors: Minamida, Kimiko Browse this author
Shiga, Kazuki Browse this author
Sujaya I., Nengah Browse this author
Sone, Teruo Browse this author
Yokota, Atsushi Browse this author
Hara, Hiroshi Browse this author →KAKEN DB
Asano, Kozo Browse this author →KAKEN DB
Tomita, Fusao Browse this author
Keywords: difructose anhydride III (DFA III)
denaturing gradient gel electrophoresis (DGGE)
Ruminococcus sp
Issue Date: Mar-2005
Publisher: 日本生物工学会
Journal Title: Journal of Bioscience and Bioengineering
Volume: 99
Issue: 3
Start Page: 230
End Page: 236
Publisher DOI: 10.1263/jbb.99.230
PMID: 16233782
Abstract: The effects of difructose anhydride III (di-D-fructofuranose-1,2′:2,3′-dianhydride; DFA III) administration (3% DFA III for 4 weeks) on rat intestinal microbiota were examined using denaturing gradient gel electrophoresis (DGGE). According to DGGE profiles, the number of bacteria related to Bacteroides acidofaciens and uncultured bacteria within the Clostridium lituseburense group decreased, while that of bacteria related to Bacteroides vulgatus, Bacteroides uniformis and Ruminococcus productus increased in DFA III-fed rat cecum. In the cecal contents of DFA III-fed rats, a lowering of pH and an increase in short chain fatty acids (SCFAs), especially acetic acid, were observed. The DFA III-assimilating bacterium, Ruminococcus sp. M-1, was isolated from the cecal contents of DFA III-fed rats. The strain had 98% similarity with R. productus ATCC 27340T (L76595), and mainly produced acetic acid. These results confirmed that the bacteria harmful to host health were not increased by DFA III administration. Moreover, DFA III stimulated the growth of Ruminococcus sp. M-1 producing acetic acid, which may alter the intestinal microbiota towards a healthier composition. It is expected that DFA III would be a new candidate as a prebiotic.
Type: article (author version)
Appears in Collections:農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 冨田 房男

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