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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Agriculture / Faculty of Agriculture >
Peer-reviewed Journal Articles, etc >
Production of equol from daidzein by gram-positive rod-shaped bacterium isolated from rat intestine.
| Title: | Production of equol from daidzein by gram-positive rod-shaped bacterium isolated from rat intestine. |
| Authors: | Minamida, Kimiko Browse this author | | Tanaka, Michiko Browse this author | | Abe, Ayumi Browse this author | | Sone, Teruo Browse this author →KAKEN DB | | Tomita, Fusao Browse this author | | Hara, Hiroshi Browse this author | | Asano, Kozo Browse this author →KAKEN DB |
| Keywords: | isoflavone | | equol | | equol-producing bacterium |
| Issue Date: | Sep-2006 |
| Publisher: | 日本生物工学会 |
| Journal Title: | Journal of Bioscience and Bioengineering |
| Volume: | 102 |
| Issue: | 3 |
| Start Page: | 247 |
| End Page: | 250 |
| Publisher DOI: | 10.1263/jbb.102.247 |
| PMID: | 17046543 |
| Abstract: | Isoflavones (mainly daidzein and genistin) belong to the flavonoid group of compounds and are classified as phytoestrogens. In the intestine, daidzin is converted to daidzein by β-glucosidase, and then daidzein is converted to O-desmethylangolensin (O-DMA) or equol via dihydrodaidzein by enzymes of intestinal bacteria. We isolated, for the first time, an anaerobic gram-positive rod-shaped strain capable of producing equol from daidzein. Its 16S rDNA gene sequence (1428 bp) showed 99% similarity with that of the human intestinal bacterium SNU-Julong 732 (AY310748) and 93% similarity with that of Eggerthella lenta ATCC 25559T (AF292375). This strain converted daidzein to equol via dihydrodaidzein in an equol-assay medium anaerobically. The addition of butyric acid and arginine increased the conversion ratio of daidzein to equol 4.7- and 4.5-fold, respectively. |
| Relation: | http://www.sbj.or.jp/ |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/14823 |
| Appears in Collections: | 農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 冨田 房男
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