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Production of equol from daidzein by gram-positive rod-shaped bacterium isolated from rat intestine.

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Title: Production of equol from daidzein by gram-positive rod-shaped bacterium isolated from rat intestine.
Authors: Minamida, Kimiko Browse this author
Tanaka, Michiko Browse this author
Abe, Ayumi Browse this author
Sone, Teruo Browse this author →KAKEN DB
Tomita, Fusao Browse this author
Hara, Hiroshi Browse this author
Asano, Kozo Browse this author →KAKEN DB
Keywords: isoflavone
equol-producing bacterium
Issue Date: Sep-2006
Publisher: 日本生物工学会
Journal Title: Journal of Bioscience and Bioengineering
Volume: 102
Issue: 3
Start Page: 247
End Page: 250
Publisher DOI: 10.1263/jbb.102.247
PMID: 17046543
Abstract: Isoflavones (mainly daidzein and genistin) belong to the flavonoid group of compounds and are classified as phytoestrogens. In the intestine, daidzin is converted to daidzein by β-glucosidase, and then daidzein is converted to O-desmethylangolensin (O-DMA) or equol via dihydrodaidzein by enzymes of intestinal bacteria. We isolated, for the first time, an anaerobic gram-positive rod-shaped strain capable of producing equol from daidzein. Its 16S rDNA gene sequence (1428 bp) showed 99% similarity with that of the human intestinal bacterium SNU-Julong 732 (AY310748) and 93% similarity with that of Eggerthella lenta ATCC 25559T (AF292375). This strain converted daidzein to equol via dihydrodaidzein in an equol-assay medium anaerobically. The addition of butyric acid and arginine increased the conversion ratio of daidzein to equol 4.7- and 4.5-fold, respectively.
Type: article (author version)
Appears in Collections:農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 冨田 房男

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