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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Agriculture / Faculty of Agriculture >
Peer-reviewed Journal Articles, etc >
The effects of di-D-fructofuranose-1,2':2,3'-dianhydride (DFA III) administration on human intestinal microbiota.
| Title: | The effects of di-D-fructofuranose-1,2':2,3'-dianhydride (DFA III) administration on human intestinal microbiota. |
| Authors: | Minamida, Kimiko Browse this author | | Sujaya I., Nengah Browse this author | | Tamura, Akiko Browse this author | | Shigematsu, Norihiro Browse this author | | Sone, Teruo Browse this author →KAKEN DB | | Yokota, Atsushi Browse this author →KAKEN DB | | Asano, Kozo Browse this author →KAKEN DB | | Benno, Yoshimi Browse this author | | Tomita, Fusao Browse this author |
| Keywords: | di-D-fructofuranose-1,2':2,3'-dianhydride (DFA III) | | human intestinal microbiota | | denaturing gradient gel electrophoresis (DGGE) |
| Issue Date: | 2004 |
| Publisher: | 日本生物工学会 |
| Journal Title: | Journal of Bioscience and Bioengineering |
| Volume: | 98 |
| Issue: | 4 |
| Start Page: | 244 |
| End Page: | 250 |
| Publisher DOI: | 10.1263/jbb.98.244 |
| PMID: | 16233700 |
| Abstract: | Di-D-fructofuranose-1,2′:2,3′-dianhydride (DFA III) was shown to enhance Ca absorption in rat and human intestine. The effects of DFA III administration (9 g per day for 4 weeks that corresponded to 3-fold the optimal dosage of DFA III) on human intestinal microbiota were studied using denaturing gradient gel electrophoresis (DGGE). The major groups of human intestinal microbiota reported previously: the Bacteroides, the Clostridium coccoides group (Clostridium cluster XIVa), the Clostridium leptum group (Clostridium cluster IV), and the Bifidobacterium group were detected. The similarity of 30 DGGE profiles based on the V3 region (before and after administration to the 15 subjects) of the 16S rDNA were calculated using Pearson’s correlation based on numbers, positions and intensity of bands, and then a dendrogram of DGGE profiles was constructed by the unweighted pair group method using arithmetic average (UPGMA) clustering method. By these analyses, no difference in DGGE profiles after DFA III administration was observed in healthy subjects, while two subjects with chronic constipation showed different profiles, namely on numbers, positions and the intensity of some bands. Their stools were softer and stool frequencies increased and they obtained relief from constipation. |
| Relation: | http://www.sbj.or.jp/ |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/14824 |
| Appears in Collections: | 農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 冨田 房男
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