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Lipid peroxidation of a human hepatoma cell line (HepG2) after incorporation of linoleic acid, arachidonic acid, and docosahexaenoic acid

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Title: Lipid peroxidation of a human hepatoma cell line (HepG2) after incorporation of linoleic acid, arachidonic acid, and docosahexaenoic acid
Authors: Araseki, Mina Browse this author
Kobayashi, Hidetaka Browse this author
Hosokawa, Masashi Browse this author →KAKEN DB
Miyashita, Kazuo Browse this author →KAKEN DB
Keywords: cellular lipid peroxidation
H2O2
isomeric distribution of monohydroperoxide
docosahexaenoic acid (DHA) conformation
Issue Date: Mar-2005
Publisher: 日本農芸化学会
Journal Title: Bioscience Biotechnology and Biochemistry
Volume: 69
Issue: 3
Start Page: 483
End Page: 490
Publisher DOI: 10.1271/bbb.69.483
PMID: 15784975
Abstract: Lipid peroxidation of human heptoma cell line, HepG2, after incorporation of linoleic acid (LA), arachidonic acid (AA), and docosahexaenoic acid (DHA) was measured with a fluorescent probe and gas chromatography–mass spectrometry (GC–MS) analysis. The analysis with a fluorescent probe showed that incorporation of each polyunsaturated fatty acid (PUFA) enhanced the cellular lipid peroxidation level, but there was little difference in the effect of LA, AA, or DHA on the enhancement of cellular lipid peroxidation. The fluorescent analysis also showed that the addition of H2O2 (0.5 mM) enhanced the cellular lipid peroxidation levels in LA and AA supplemented cells as compared with those without H2O2. However, the enhancement of lipid peroxidation by H2O2 was not observed in DHA-supplemented cells. The same result was obtained in the GC–MS analysis of total amounts of monohydroperoxides (MHP) formed in the cellular phospholipid oxidation. In this case, the main source for MHP was LA in LA-, AA-, and DHA-supplemented cells. A significant amount of AA–MHP and a small amount of DHA–MHP were observed in AA- and DHA-supplemented cells respectively. GC–MS analysis also indicated the specific positional distribution of DHA–MHP isomers. The isomers were formed only by hydrogen abstraction at the C-18 (16-MHP + 20-MHP; 46.5%), C-6 (4-MHP + 8-MHP; 38.5%), and C-12 (10-MHP + 14-MHP; 15.1%) positions, but not at the C-9 or C-15 positions.
Relation: http://www.jstage.jst.go.jp/
Type: article
URI: http://hdl.handle.net/2115/14828
Appears in Collections:水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 宮下 和夫

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