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Recombinant production of docosahexaenoic acid in a polyketide biosynthesis mode in Escherichia coli.
Title: | Recombinant production of docosahexaenoic acid in a polyketide biosynthesis mode in Escherichia coli. |
Authors: | Orikasa, Yoshitake Browse this author | Nishida, Takanori Browse this author | Yamada, Akiko Browse this author | Yu, Reiko Browse this author | Watanabe, Kazuo Browse this author | Hase, Akira Browse this author | Morita, Naoki Browse this author | Okuyama, Hidetoshi Browse this author |
Keywords: | DHA biosynthesis genes | Docosahexaenoic acid | Eicosapentaenoic acid | Moritella marina strain MP-1 | Polyunsaturated fatty acids |
Issue Date: | Nov-2006 |
Publisher: | Springer |
Journal Title: | Biotechnology Letters |
Volume: | 28 |
Issue: | 22 |
Start Page: | 1841 |
End Page: | 1847 |
Publisher DOI: | 10.1007/s10529-006-9168-6 |
PMID: | 16988784 |
Abstract: | The docosahexaenoic acid (DHA) biosynthesis gene cluster (pDHA3) from the DHA-producing Moritella marina strain MP-1 includes the genes pfaA, pfaB, pfaC, and pfaD, which are similar to the genes of polyketide biosynthesis. When this cluster was co-expressed in Escherichia coli with M. marina MP-1 pfaE, which encodes phosphopantetheinyl transferase, DHA was biosynthesized. The maximum production of DHA (5% of total fatty acids) was observed at 15°C. This is the first report of the recombinant production of DHA in a polyketide biosynthesis mode. |
Rights: | The original publication is available at www.springerlink.com |
Type: | article (author version) |
URI: | http://hdl.handle.net/2115/14890 |
Appears in Collections: | 環境科学院・地球環境科学研究院 (Graduate School of Environmental Science / Faculty of Environmental Earth Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 奥山 英登志
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