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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Environmental Science / Faculty of Environmental Earth Science >
Peer-reviewed Journal Articles, etc >
Recombinant production of docosahexaenoic acid in a polyketide biosynthesis mode in Escherichia coli.
| Title: | Recombinant production of docosahexaenoic acid in a polyketide biosynthesis mode in Escherichia coli. |
| Authors: | Orikasa, Yoshitake Browse this author | | Nishida, Takanori Browse this author | | Yamada, Akiko Browse this author | | Yu, Reiko Browse this author | | Watanabe, Kazuo Browse this author | | Hase, Akira Browse this author | | Morita, Naoki Browse this author | | Okuyama, Hidetoshi Browse this author |
| Keywords: | DHA biosynthesis genes | | Docosahexaenoic acid | | Eicosapentaenoic acid | | Moritella marina strain MP-1 | | Polyunsaturated fatty acids |
| Issue Date: | Nov-2006 |
| Publisher: | Springer |
| Journal Title: | Biotechnology Letters |
| Volume: | 28 |
| Issue: | 22 |
| Start Page: | 1841 |
| End Page: | 1847 |
| Publisher DOI: | 10.1007/s10529-006-9168-6 |
| PMID: | 16988784 |
| Abstract: | The docosahexaenoic acid (DHA) biosynthesis gene cluster (pDHA3) from the DHA-producing Moritella marina strain MP-1 includes the genes pfaA, pfaB, pfaC, and pfaD, which are similar to the genes of polyketide biosynthesis. When this cluster was co-expressed in Escherichia coli with M. marina MP-1 pfaE, which encodes phosphopantetheinyl transferase, DHA was biosynthesized. The maximum production of DHA (5% of total fatty acids) was observed at 15°C. This is the first report of the recombinant production of DHA in a polyketide biosynthesis mode. |
| Rights: | The original publication is available at www.springerlink.com |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/14890 |
| Appears in Collections: | 環境科学院・地球環境科学研究院 (Graduate School of Environmental Science / Faculty of Environmental Earth Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 奥山 英登志
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