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Recombinant production of docosahexaenoic acid in a polyketide biosynthesis mode in Escherichia coli.

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Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/14890

Title: Recombinant production of docosahexaenoic acid in a polyketide biosynthesis mode in Escherichia coli.
Authors: Orikasa, Yoshitake Browse this author
Nishida, Takanori Browse this author
Yamada, Akiko Browse this author
Yu, Reiko Browse this author
Watanabe, Kazuo Browse this author
Hase, Akira Browse this author
Morita, Naoki Browse this author
Okuyama, Hidetoshi Browse this author
Keywords: DHA biosynthesis genes
Docosahexaenoic acid
Eicosapentaenoic acid
Moritella marina strain MP-1
Polyunsaturated fatty acids
Issue Date: Nov-2006
Publisher: Springer
Journal Title: Biotechnology Letters
Volume: 28
Issue: 22
Start Page: 1841
End Page: 1847
Publisher DOI: 10.1007/s10529-006-9168-6
PMID: 16988784
Abstract: The docosahexaenoic acid (DHA) biosynthesis gene cluster (pDHA3) from the DHA-producing Moritella marina strain MP-1 includes the genes pfaA, pfaB, pfaC, and pfaD, which are similar to the genes of polyketide biosynthesis. When this cluster was co-expressed in Escherichia coli with M. marina MP-1 pfaE, which encodes phosphopantetheinyl transferase, DHA was biosynthesized. The maximum production of DHA (5% of total fatty acids) was observed at 15°C. This is the first report of the recombinant production of DHA in a polyketide biosynthesis mode.
Rights: The original publication is available at www.springerlink.com
Type: article (author version)
URI: http://hdl.handle.net/2115/14890
Appears in Collections:環境科学院・地球環境科学研究院 (Graduate School of Environmental Science / Faculty of Environmental Earth Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 奥山 英登志

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