HUSCAP logo Hokkaido Univ. logo

Hokkaido University Collection of Scholarly and Academic Papers >
水産科学院・水産科学研究院  >
雑誌発表論文等  >

Trypsins from the pyloric ceca of jacopever (Sebastes schlegelii) and elkhorn sculpin (Alcichthys alcicornis): Isolation and characterization

フルテキスト
FOCH100-4.pdf185.35 kBPDF見る/開く
この文献へのリンクには次のURLを使用してください:http://hdl.handle.net/2115/14904

タイトル: Trypsins from the pyloric ceca of jacopever (Sebastes schlegelii) and elkhorn sculpin (Alcichthys alcicornis): Isolation and characterization
著者: Kishimura, Hideki 著作を一覧する
Tokuda, Yusuke 著作を一覧する
Yabe, Mamoru 著作を一覧する
Klomklao, Sappasith 著作を一覧する
Benjakul, Soottawat 著作を一覧する
Ando, Seiichi 著作を一覧する
キーワード: Jacopever
Sebastes schlegelii
Elkhorn sculpin
Alcichthys alcicornis
Pyloric ceca
Trypsin
Characteristics
発行日: 2007年
出版者: Elsevier B.V.
誌名: Food Chemistry
巻: 100
号: 4
開始ページ: 1490
終了ページ: 1495
出版社 DOI: 10.1016/j.foodchem.2005.11.040
抄録: Laboratory of Marine Products and Food Science, Research Faculty of Fisheries Sciences, Hokkaido University, Hakodate, Hokkaido 041-8611, Japan bDepartment of Food Technology, Faculty of Agro-Industry, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand cDepartment of Fisheries Science, Faculty of Fisheries, Kagoshima University, Shimoarata, Kagoshima 890-0056, Japan Received 5 September 2005; accepted 23 November 2005. Available online 7 February 2006. Abstract Trypsins from the pyloric ceca of jacopever (Sebastes schlegelii), TR-J, and elkhorn sculpin (Alcichthys alcicornis), TR-E, were purified by gel filtration on Sephacryl S-200 and Sephadex G-50. The molecular weights of TR-J and TR-E were estimated to be 24,000 Da by sodium dodecyl sulfate–polyacrylamide gel electrophoresis. TR-J and TR-E revealed optimum temperatures of 60 and 50 °C, respectively, and showed the same optimum pH (pH 8.0) for hydrolysis of Nα-p-tosyl-l-arginine methyl ester. TR-J and TR-E were unstable at above 50 and 40 °C, respectively, and were more stable at alkaline pH than at acidic pH. Thermal stabilities of TR-J and TR-E were highly calcium dependent. These purified trypsin enzymes were inhibited by serine protease inhibitors, such as TLCK and soybean trypsin inhibitor. The N-terminal amino acid sequences of TR-J and TR-E were also investigated. The N-terminal amino acid sequences of TR-J, IVGGYECKPYSQPHQVSLNS, and TR-E, IVGGYECTPHSQAHQVSLNS, were found, and these sequences showed highly homology to other fish trypsins. Keywords: Jacopever; Sebastes schlegelii; Elkhorn sculpin; Alcichthys alcicornis; Pyloric ceca; Trypsin; Characteristics
Relation (URI): http://www.sciencedirect.com/science/journal/03088146
資料タイプ: article (author version)
URI: http://hdl.handle.net/2115/14904
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 岸村 栄毅

 

本サイトに関するご意見・お問い合わせは repo at lib.hokudai.ac.jp へお願いします。 - 北海道大学