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Trypsins from the pyloric ceca of jacopever (Sebastes schlegelii) and elkhorn sculpin (Alcichthys alcicornis): Isolation and characterization

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Title: Trypsins from the pyloric ceca of jacopever (Sebastes schlegelii) and elkhorn sculpin (Alcichthys alcicornis): Isolation and characterization
Authors: Kishimura, Hideki Browse this author →KAKEN DB
Tokuda, Yusuke Browse this author
Yabe, Mamoru Browse this author →KAKEN DB
Klomklao, Sappasith Browse this author
Benjakul, Soottawat Browse this author
Ando, Seiichi Browse this author
Keywords: Jacopever
Sebastes schlegelii
Elkhorn sculpin
Alcichthys alcicornis
Pyloric ceca
Trypsin
Characteristics
Issue Date: 2007
Publisher: Elsevier B.V.
Journal Title: Food Chemistry
Volume: 100
Issue: 4
Start Page: 1490
End Page: 1495
Publisher DOI: 10.1016/j.foodchem.2005.11.040
Abstract: Trypsins from the pyloric ceca of jacopever (Sebastes schlegelii), TR-J, and elkhorn sculpin (Alcichthys alcicornis), TR-E, were purified by gel filtration on Sephacryl S-200 and Sephadex G-50. The molecular weights of TR-J and TR-E were estimated to be 24,000 Da by sodium dodecyl sulfate–polyacrylamide gel electrophoresis. TR-J and TR-E revealed optimum temperatures of 60 and 50 °C, respectively, and showed the same optimum pH (pH 8.0) for hydrolysis of Nα-p-tosyl-l-arginine methyl ester. TR-J and TR-E were unstable at above 50 and 40 °C, respectively, and were more stable at alkaline pH than at acidic pH. Thermal stabilities of TR-J and TR-E were highly calcium dependent. These purified trypsin enzymes were inhibited by serine protease inhibitors, such as TLCK and soybean trypsin inhibitor. The N-terminal amino acid sequences of TR-J and TR-E were also investigated. The N-terminal amino acid sequences of TR-J, IVGGYECKPYSQPHQVSLNS, and TR-E, IVGGYECTPHSQAHQVSLNS, were found, and these sequences showed highly homology to other fish trypsins.
Relation: http://www.sciencedirect.com/science/journal/03088146
Type: article (author version)
URI: http://hdl.handle.net/2115/14904
Appears in Collections:水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 岸村 栄毅

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