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Acquisition and transmission of Theileria parva by vector tick, Rhipicephalus appendiculatus

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タイトル: Acquisition and transmission of Theileria parva by vector tick, Rhipicephalus appendiculatus
著者: Konnai, Satoru 著作を一覧する
Imamura, Saiki 著作を一覧する
Nakajima, Chie 著作を一覧する
Witola, William Harold 著作を一覧する
Yamada, Shinji 著作を一覧する
Simuunza, Martin 著作を一覧する
Nambota, Andrew 著作を一覧する
Yasuda, Jun 著作を一覧する
Ohashi, Kazuhiko 著作を一覧する
Onuma, Misao 著作を一覧する
キーワード: Theileria parva
Real-time PCR
Detection
Monitoring
Transmission
発行日: 2006年 8月
出版者: Elsevier B.V.
誌名: Acta Tropica
巻: 99
号: 1
開始ページ: 34
終了ページ: 41
出版社 DOI: 10.1016/j.actatropica.2006.06.008
抄録: In order to investigate the transmission dynamics of Theileria parva (T. parva) by the brown ear tick, Rhipicephalus appendiculatus (R. appendiculatus), under experimental conditions, detection of T. parva in ticks and cattle was performed by a quantitative real-time PCR assay. A calf inoculated with a T. parva mixture became PCR-positive for T. parva infection on day 8 post-inoculation, and subsequently, nymphal ticks were introduced and maintained to feed on the infected calf for 6 days. Engorged nymphs were collected daily and allowed to molt into adults, and overall, 70.8% (121/171) of the adult ticks acquired the T. parva infection. Furthermore, the T. parva infection rate in ticks under field conditions was monitored by real-time PCR in R. appendiculatus ticks collected from a traditionally managed pastoral land of Zambia, on which Sanga breed cattle are traditionally reared and the area has endemic East Coast fever (ECF). A total of 70 cattle were randomly selected in the same area and 67 (95.7%) were found to be serologically positive for R. appendiculatus tick antigen (RIM36). Twenty-nine (43.3%) of the 67 serologically positive cattle were real-time PCR-positive for T. parva, although no piroplasms could be detected in the blood smears. Unexpectedly, out of 614 R. appendiculatus nymphal and adult ticks collected by flagging vegetation, 4.1% were positive for T. parva DNA. However, since the rate of transmission of T. parva from infected cattle to ticks and vice versa and the serological evidence of exposure to R. appendiculatus ticks in naturally exposed cattle were relatively high, it would be wise in such a case to consider vector control as well as vaccination against ECF as control measures.
Relation (URI): http://www.sciencedirect.com/science/journal/0001706X
資料タイプ: article (author version)
URI: http://hdl.handle.net/2115/15427
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

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