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A method of cell-sheet preparation using collagenase digestion of salmon atelocollagen fibrillar gel.

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タイトル: A method of cell-sheet preparation using collagenase digestion of salmon atelocollagen fibrillar gel.
著者: Nagai, Nobuhiro 著作を一覧する
Yunoki, Shunji 著作を一覧する
Satoh, Yasuharu 著作を一覧する
Tajima, Kenji 著作を一覧する
Munekata, Masanobu 著作を一覧する
キーワード: cell sheet
salmon atelocollagen
fibrillar gel
collagenase digestion
発行日: 2004年
出版者: Society for Bioscience and Bioengineering, Japan
誌名: Journal of Bioscience and Bioengineering
巻: 98
号: 6
開始ページ: 493
終了ページ: 496
出版社 DOI: 10.1016/S1389-1723(05)00319-1
抄録: We prepared a cell sheet by using collagenase treatment to digest salmon atelocollagen fibrillar gel (SAC gel) on which human periodontal ligament (HPDL) cells had been cultured. The SAC gel was found to be digested completely within 2 h at a concentration of 50 U of collagenase per mg of collagen. The SAC gel on which HPDL cells were cultured for 10 d was treated with collagenase, resulting in the formation of a detached and shrunken cell sheet. Immunostaining results showed that the cytoskeleton and fibronectin matrix level of the cell sheet were maintained after collagenase treatment. In addition, collagenase treatment had almost no effect on the activities of HPDL cells.
Relation (URI): http://www.sbj.or.jp/
http://www.sciencedirect.com/science/journal/13891723
資料タイプ: article (author version)
URI: http://hdl.handle.net/2115/15816
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 永井 展裕

 

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