HUSCAP logo Hokkaido Univ. logo

Hokkaido University Collection of Scholarly and Academic Papers >
Creative Research Institution >
Peer-reviewed Journal Articles, etc >

A method of cell-sheet preparation using collagenase digestion of salmon atelocollagen fibrillar gel.

Files in This Item:
JBB98-6.pdf737.53 kBPDFView/Open
Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/15816

Title: A method of cell-sheet preparation using collagenase digestion of salmon atelocollagen fibrillar gel.
Authors: Nagai, Nobuhiro Browse this author
Yunoki, Shunji Browse this author
Satoh, Yasuharu Browse this author →KAKEN DB
Tajima, Kenji Browse this author →KAKEN DB
Munekata, Masanobu Browse this author
Keywords: cell sheet
salmon atelocollagen
fibrillar gel
collagenase digestion
Issue Date: 2004
Publisher: Society for Bioscience and Bioengineering, Japan
Journal Title: Journal of Bioscience and Bioengineering
Volume: 98
Issue: 6
Start Page: 493
End Page: 496
Publisher DOI: 10.1016/S1389-1723(05)00319-1
PMID: 16233743
Abstract: We prepared a cell sheet by using collagenase treatment to digest salmon atelocollagen fibrillar gel (SAC gel) on which human periodontal ligament (HPDL) cells had been cultured. The SAC gel was found to be digested completely within 2 h at a concentration of 50 U of collagenase per mg of collagen. The SAC gel on which HPDL cells were cultured for 10 d was treated with collagenase, resulting in the formation of a detached and shrunken cell sheet. Immunostaining results showed that the cytoskeleton and fibronectin matrix level of the cell sheet were maintained after collagenase treatment. In addition, collagenase treatment had almost no effect on the activities of HPDL cells.
Relation: http://www.sbj.or.jp/
http://www.sciencedirect.com/science/journal/13891723
Type: article (author version)
URI: http://hdl.handle.net/2115/15816
Appears in Collections:創成研究機構 (Creative Research Institution) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 永井 展裕

Export metadata:

OAI-PMH ( junii2 , jpcoar )

MathJax is now OFF:


 

 - Hokkaido University