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Relationship between adsorbed fibronectin and cell adhesion on a honeycomb-patterned film

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Title: Relationship between adsorbed fibronectin and cell adhesion on a honeycomb-patterned film
Authors: Yamamoto, Sadaaki Browse this author
Tanaka, Masaru Browse this author
Sunami, Hiroshi Browse this author
Arai, Keiko Browse this author
Takayama, Aiko Browse this author
Yamashita, Shigeko Browse this author
Morita, Yuka Browse this author
Shimomura, Masatsugu Browse this author
Keywords: Atomic force microscopy
Scanning electron microscopy
Cell adhesion
Surface topography
Tissue engineering
Issue Date: 15-Sep-2006
Publisher: Elsevier B.V.
Journal Title: Surface Science
Volume: 600
Issue: 18
Start Page: 3785
End Page: 3791
Publisher DOI: 10.1016/j.susc.2006.01.085
Abstract: Substratum surface morphology plays a vital roles in cellular behavior. Here, we characterized adsorption of fibronectin (Fn) as a typical cell adhesion protein onto honeycomb-patterned films made of poly(ε-caprolactone) (PCL) by using atomic force microscopy (AFM) and confocal laser scanning microscopy (CLSM). In order to determine how cells adhere to a honeycomb-patterned film, focal adhesion of cardiac myocytes (CMYs) and endothelial cells (ECs) on the films were studied by using fluorescence labeling of vinculin. Fn adsorbs around the pore edges to form ring-shaped structures. CMYs and ECs adhere onto the honeycomb-patterned films at focal contact points localized around pore edges distributed over the entire cellular surface. The focal contact points on the honeycomb-patterned films correspond well with the adsorption sites of Fn. We suggest that the cell response to honeycomb-patterned films is associated with the adsorption pattern of Fn on the film.
Type: article (author version)
Appears in Collections:創成研究機構 (Creative Research Institution) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 山本 貞明

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