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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Medicine / Faculty of Medicine >
Peer-reviewed Journal Articles, etc >
Establishment and characterization of amylase-producing lung adenocarcinoma cell line, IMEC-2.
| Title: | Establishment and characterization of amylase-producing lung adenocarcinoma cell line, IMEC-2. |
| Authors: | Yokouchi, Hiroshi Browse this author | | Yamazaki, Koichi Browse this author | | Asahina, Hajime Browse this author | | Shigemura, Masahiko Browse this author | | Moriyama, Takanori Browse this author | | Takaoka, Kazuo Browse this author | | Moriya, Jun Browse this author | | Itoh, Tomoo Browse this author | | Kinoshita, Ichiro Browse this author | | Dosaka-Akita, Hirotoshi Browse this author | | Tsutsumi, Yutaka Browse this author | | Nishimura, Masaharu Browse this author |
| Keywords: | amylase | | lung cancer | | cell line | | sialylation | | IMEC-2 |
| Issue Date: | Jul-2006 |
| Volume: | 26 |
| Issue: | 4B |
| Start Page: | 2821 |
| End Page: | 2827 |
| PMID: | 16886600 |
| Abstract: | Background: Few studies have successfully established an amylase-producing lung cancer cell line or have examined its cytological, biochemical and biological features. Materials and Methods: Cancer cells isolated from pleural effusion using a gradient method were cultivated. Results: Amylase production from the newly established cell line was confirmed by positive staining for α-amylase and increased amylase levels in culture supernatant. Electron microscopy revealed zymogen granule-like structures. Sialylation of salivary-type amylase was confirmed directly from the cell line by examining neuraminidase sensitivity and amylase elution profile under high-performance liquid chromatography. EGFR or KRAS mutation was not found. Conclusions: This cell line offers a useful tool for analyzing the pathogenesis and pathophysiology of amylase-producing lung cancers. Moreover, it might be useful for probing metastasis and invasiveness of lung cancer cells and for developing an early diagnostic method based on sialylated salivary-amylase production. |
| Relation: | http://www.amedeo.com/medicine/ln/ln4.htm |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/15843 |
| Appears in Collections: | 医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 西村 正治
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