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Establishment and characterization of amylase-producing lung adenocarcinoma cell line, IMEC-2.

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タイトル: Establishment and characterization of amylase-producing lung adenocarcinoma cell line, IMEC-2.
著者: Yokouchi, Hiroshi 著作を一覧する
Yamazaki, Koichi 著作を一覧する
Asahina, Hajime 著作を一覧する
Shigemura, Masahiko 著作を一覧する
Moriyama, Takanori 著作を一覧する
Takaoka, Kazuo 著作を一覧する
Moriya, Jun 著作を一覧する
Itoh, Tomoo 著作を一覧する
Kinoshita, Ichiro 著作を一覧する
Dosaka-Akita, Hirotoshi 著作を一覧する
Tsutsumi, Yutaka 著作を一覧する
Nishimura, Masaharu 著作を一覧する
キーワード: amylase
lung cancer
cell line
発行日: 2006年 7月
巻: 26
号: 4B
開始ページ: 2821
終了ページ: 2827
抄録: Background: Few studies have successfully established an amylase-producing lung cancer cell line or have examined its cytological, biochemical and biological features. Materials and Methods: Cancer cells isolated from pleural effusion using a gradient method were cultivated. Results: Amylase production from the newly established cell line was confirmed by positive staining for α-amylase and increased amylase levels in culture supernatant. Electron microscopy revealed zymogen granule-like structures. Sialylation of salivary-type amylase was confirmed directly from the cell line by examining neuraminidase sensitivity and amylase elution profile under high-performance liquid chromatography. EGFR or KRAS mutation was not found. Conclusions: This cell line offers a useful tool for analyzing the pathogenesis and pathophysiology of amylase-producing lung cancers. Moreover, it might be useful for probing metastasis and invasiveness of lung cancer cells and for developing an early diagnostic method based on sialylated salivary-amylase production.
Relation (URI):
資料タイプ: article (author version)
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 西村 正治


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