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Establishment and characterization of amylase-producing lung adenocarcinoma cell line, IMEC-2.

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Title: Establishment and characterization of amylase-producing lung adenocarcinoma cell line, IMEC-2.
Authors: Yokouchi, Hiroshi Browse this author
Yamazaki, Koichi Browse this author
Asahina, Hajime Browse this author
Shigemura, Masahiko Browse this author
Moriyama, Takanori Browse this author
Takaoka, Kazuo Browse this author
Moriya, Jun Browse this author
Itoh, Tomoo Browse this author
Kinoshita, Ichiro Browse this author
Dosaka-Akita, Hirotoshi Browse this author
Tsutsumi, Yutaka Browse this author
Nishimura, Masaharu Browse this author
Keywords: amylase
lung cancer
cell line
Issue Date: Jul-2006
Volume: 26
Issue: 4B
Start Page: 2821
End Page: 2827
PMID: 16886600
Abstract: Background: Few studies have successfully established an amylase-producing lung cancer cell line or have examined its cytological, biochemical and biological features. Materials and Methods: Cancer cells isolated from pleural effusion using a gradient method were cultivated. Results: Amylase production from the newly established cell line was confirmed by positive staining for α-amylase and increased amylase levels in culture supernatant. Electron microscopy revealed zymogen granule-like structures. Sialylation of salivary-type amylase was confirmed directly from the cell line by examining neuraminidase sensitivity and amylase elution profile under high-performance liquid chromatography. EGFR or KRAS mutation was not found. Conclusions: This cell line offers a useful tool for analyzing the pathogenesis and pathophysiology of amylase-producing lung cancers. Moreover, it might be useful for probing metastasis and invasiveness of lung cancer cells and for developing an early diagnostic method based on sialylated salivary-amylase production.
Type: article (author version)
Appears in Collections:医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 西村 正治

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