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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Agriculture / Faculty of Agriculture >
Peer-reviewed Journal Articles, etc >
Soft gel medium solidified with gellan gum for preliminary screening for root-associating, free-living nitrogen-fixing bacteria inhabiting the rhizoplane of plants
| Title: | Soft gel medium solidified with gellan gum for preliminary screening for root-associating, free-living nitrogen-fixing bacteria inhabiting the rhizoplane of plants |
| Authors: | Hashidoko, Yasuyuki Browse this author →KAKEN DB | | Tada, Motohiko Browse this author | | Osaki, Mitsuru Browse this author →KAKEN DB | | Tahara, Satoshi Browse this author |
| Keywords: | free-living nitrogen-fixing bacterium | | gellan gum | | Plantago lanceolata | | rhizoplane microflora |
| Issue Date: | Oct-2002 |
| Publisher: | Japan Society for Bioscience, Biotechnology, and Agrochemistry |
| Journal Title: | Bioscience Biotechnology and Biochemistry |
| Volume: | 66 |
| Issue: | 10 |
| Start Page: | 2259 |
| End Page: | 2263 |
| Publisher DOI: | 10.1271/bbb.66.2259 |
| PMID: | 12450146 |
| Abstract: | For preliminary screening for and characterization of free-living nitrogen-fixing bacteria from rhizoplane microflora, we used Winogradsky's mineral mixture-based nitrogen-free medium solidified with 0.3% gellan gum. The soft gel medium enabled some reference and wild free-living nitrogen-fixing bacteria to grow in characteristic colonies, including their reaction to oxygen and their motility change. Gellan gum is thus likely to be a better gel matrix than agarose for the investigation of root-associating, free-living nitrogen-fixing bacteria to identify their characteristic behaviors. |
| Relation: | http://www.jstage.jst.go.jp/ |
| Type: | article |
| URI: | http://hdl.handle.net/2115/15846 |
| Appears in Collections: | 農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 橋床 泰之
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